Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel

Author(s)

Braga, Aline Silva ^[1] ; Simas, Leticia Lobo de Melo ^[1] ; Pires, Juliana Gonsalves ^[1] ; Souza, Beatriz Martines ^[1] ; Melo, Fernanda Pereira de Souza Rosa de ^[2] ; Saldanha, Luiz Leonardo ^[2] ; Dokkedal, Anne Ligia ^[2] ; Magalhaes, Ana Carolina ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Sci, Dept Biol Sci, Bauru, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 99, AUG 2020.
• Web of Science Citations: 0

Abstract

Objective: This study evaluated the antibiofilm and anti-caries effects of an experimental mouth rinse containing aqueous extract of Matricaria chamomilla L. Methods: Microcosm biofilm was produced on bovine enamel, from pooled human saliva mixed with McBain saliva, under 0.2 % sucrose exposure, for 5 days. The biofilm was daily treated using (1 mL/1 min): Vochysia tucanorum Mart. (2.5 mg/mL); Myrcia bella Cambess. (1.25 mg/mL); Matricaria chamomilla L. (20 mg/mL); Malva sylvestris (Malvatricin (R) Plus-Daudt); 0.12 % Chlorhexidine (PerioGard (R)-Palmolive, Positive control) and PBS (Negative control). The % dead bacteria, biofilm thickness, EPS biovolume, lactic acid concentration, the CFU counting (total microorganisms, Lactobacillus sp., total streptococci and Streptococcus mutans/S. sobrinus) were determined. Enamel demineralization was measured by TMR. Results: All mouth rinses induced bacterial death compared to PBS (p < 0.0001). The biofilm thickness varied from 12 +/- 2 mu m (chlorhexidine) to 18 +/- 2 mu m (V. tucanorum) (ANOVA/Tukey, p < 0.0001). The EPS biovolume varied from 7(4)% (chlorhexidine) to 30(20)% (PBS) (Kruskal-Wallis/Dunn, p < 0.0001). The lactic acid production was reduced by M. sylvestris (1.1 +/- 0.2 g/L) and chlorhexidine (0.6 +/- 0.2 g/L) compared to PBS (2.6 +/- 1.3 g/L) (ANOVA, p < 0.0001). Malva sylvestris and chlorhexidine showed significant low CFU for total microorganisms, Lactobacillus sp. and total streptococci. Only chlorhexidine significantly reduced S. mutans/S. sobrinus. CFUs for total streptococci and Lactobacillus sp, were also significantly reduced by M. chamomilla L. Malva sylvestris (63.4 % of mineral loss reduction), chlorhexidine (47.4 %) and M. chamomilla L. (39.4 %) significantly reduced enamel demineralization compared to PBS (ANOVA/Tukey, p < 0.0001). Conclusion: M. chamomilla L. has lower antibiofilm action, but comparable anti-caries effect to those found for chlorhexidine, under this model. (AU)

• FAPESP's process: 17/00556-0 - Evaluation of the antimicrobial effect of alternative and commercial agents on a microcosm biofilm and on prevention of dental demineralization
• Grantee: Ana Carolina Magalhães
• Support type: Regular Research Grants
• FAPESP's process: 17/17249-2 - Evaluation of the effect of different experimental and commercial natural extracts in the form of toothpastes and oral mouthrinses on the viability and activity of microcosm biofilm and on enamel demineralization prevention in vitro
• Grantee: Aline Silva Braga
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/23165-6 - Evaluation of the effect of different natural extracts in the form of experimental and commercial oral mouthrinses on the viability of microcosm biofilm and on enamel demineralization prevention
• Grantee: Leticia Lobo de Melo Simas
• Support type: Scholarships in Brazil - Scientific Initiation