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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Incidence and characteristics of methicillin-resistant coagulase-negativeStaphylococcus aureusin peritoneal dialysis-associated peritonitis in a single center using molecular methods

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Camargo, Carlos Henrique [1, 2, 3] ; Ribeiro de Souza da Cunha, Maria de Lourdes [2] ; Teixeira Caramori, Jacqueline Costa [1] ; Mondelli, Alessandro Lia [1] ; Montelli, Augusto Cezar [1, 2] ; Barretti, Pasqual [1]
Total Authors: 6
[1] Sao Paulo State Univ, Botucatu Med Sch, Dept Internal Med, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Biosci Inst Botucatu, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[3] Adolfo Lutz Inst, Bacteriol Div, Ave Dr Arnaldo, 351-9 Andar, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 1

Purpose Peritonitis is a serious complication of peritoneal dialysis and coagulase-negativeStaphylococcus(CNS) is the most frequent cause of peritoneal dialysis (PD)-infections in many centers. This study aimed to investigate the molecular epidemiology of CNS isolated from PD-peritonitis in a Brazilian single center, focusing on the genetic determinants conferring methicillin resistance. Methods Bacterial strains were isolated from peritoneal fluid of patients presenting PD-peritonitis, identified by phenotypic and molecular methods, and those identified as CNS were submitted tomecAdetection, SCCmec, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results Over the 18-year period of this study (1995-2011), a total of 878 peritonitis episodes were diagnosed in this unit, 115 were caused by coagulase-negative staphylococci of which 72 byStaphylococcus epidermidis.mecAgene was detected in 55 CNS (47.8%), more frequently on the more recent years. SCCmectype III was the most frequent cassette, followed by SCCmectype IV and SCCmectype II. A diverstity of pulsotypes was observed among theS. epidermidisisolates, but five clusters (based on the 80% cutoff) were identified. Diversified sequence types (ST02, ST05, ST06, ST09, ST23, ST59 and ST371) were detected. Conclusions Detection of SCCmectype III among coagulase-negativeStaphylococcusunderscores the role of hospital environments as potential source of methicillin-resistantStaphylococcuscausing peritonitis in PD patients. (AU)

FAPESP's process: 09/15727-8 - Clonal profile and pathogenicity factors of Staphylococcus spp. on the prognostic of peritonitis in peritoneal dialysis
Grantee:Pasqual Barretti
Support type: Regular Research Grants
FAPESP's process: 09/12052-0 - Clonal profile and pathogenicity factors of Staphylococcus spp. on the prognostic of peritonitis in peritoneal dialysis
Grantee:Carlos Henrique Camargo
Support type: Scholarships in Brazil - Master