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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination

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Kassab, Giulia [1] ; Cheburkanov, Vsevolod [2] ; Willis, Jace [2] ; Moule, Madeleine G. [3] ; Kurachi, Cristina [1] ; Yakovlev, Vladislav [2] ; Cirillo, Jeffrey D. [3] ; Bagnato, Vanderlei S. [1, 4]
Total Authors: 8
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos - Brazil
[2] Texas A&M Univ, Dept Biomed Engn, College Stn, TX - USA
[3] Texas A&M Univ, Coll Med, Dept Microbial Pathogenesis & Immunol, Bryan, TX - USA
[4] Texas A&M Univ, Hagler Inst Adv Studies, College Stn, TX - USA
Total Affiliations: 4
Document type: Journal article
Source: Journal of Biophotonics; AUG 2020.
Web of Science Citations: 0

Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing ofStaphylococcus aureus(of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused byS. aureusand other important pathogens. (AU)

FAPESP's process: 18/18188-0 - Photodynamic inactivation of Bacterial Pneumonia with nebulization of the photosensitizer and extracorporeal illumination
Grantee:Giulia Kassab
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC