| Full text | |
| Author(s): Show less - |
Passarelli, Marina da Silva
[1]
;
Pinoti Pavaneli, Ana Paula
[1]
;
Ravagnani, Gisele Mouro
[1]
;
Martins, Matheus Pasini
[1]
;
Pedrosa, Ana Carolina
[1]
;
Massami Kitamura Martins, Simone Maria
[2]
;
de Alcantra Rocha, Nathalia Raissa
[3]
;
Bittar Rigo, Victor Henrique
[1]
;
Yasui, George
[3]
;
Yeste, Marc
[4]
;
Cesar de Andrade, Andre Furugen
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl & Technol Swine Embryos, Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, SP - Brazil
[3] Lab Biotechnol Fishs CEPTA ICMBio, Pirassununga, SP - Brazil
[4] Univ Girona, Biotechnol Anim & Human Reprod TechnoSperm, Unit Cell Biol, Dept Biol, Inst Food & Agr Technol, Fac Sci, Girona - Spain
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Animal Reproduction Science; v. 219, AUG 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Equilibration time (ET) is the period during which sperm cells are in contact with cooling/freezing media components at a temperature of 5 degrees C, providing a proper osmotic balance between the intra- and extra-cellular milieu. The present study aimed to determine the ET (0, 2, and 4 h) that results in greater post-thaw sperm quality and functions. Based on the post-thaw sperm membrane integrity and motility ratios, 20 ejaculates collected from five boars were classified as having good (GFE, n = 5) or poor (PFE, n = 15) freezing capacity. Ratios of post-thaw sperm with intact plasma membrane and acrosome were similar between ET (0 h: 37.02 % +/- 2.85 %; 2 h: 34.59 % +/- 7.12 %; 4 h: 37.87 % +/- 4.44 %) in GFE samples. In PFE, ratios of sperm with intact plasma membrane and acrosome at post-thaw were greater (P < 0.05) after an ET of 2 h than after an ET of 0 h (2 h: 26.16 % +/- 1.54 % and 0 h: 16.74 % +/- 1.59 %). Also, ratios of post-thaw sperm with relatively lesser membrane lipids disorder were greater (P < 0.05) after an ET of 2 h than for other ET in both GFE (2 h: 21.97 % +/- 4.24 % and 0 h: 16.63 % +/- 2.38 %) and PFE (2 h: 16.65 % +/- 1.40 % and 0 h: 13.23 % +/- 1.25 %) samples. In conclusion, an ET of 2 h results in greater sperm cryotolerance in both GFE and PFE samples, which suggests that modifying the freezing protocol lead to an increase post-thaw sperm function and survival. (AU) | |
| FAPESP's process: | 16/24690-4 - Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance |
| Grantee: | André Furugen Cesar de Andrade |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/01124-1 - Holding time and equilibration time: Its effects on cryopreserved boar spermatozoa |
| Grantee: | Marina da Silva Passarelli Zanatta |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 17/10821-2 - Evaluation of equilibration time on boar semen cryopreservation protocols: a physiological and morphofunctional study |
| Grantee: | Marina da Silva Passarelli Zanatta |
| Support Opportunities: | Scholarships in Brazil - Master |