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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Decreased Mitochondrial Function, Biogenesis, and Degradation in Peripheral Blood Mononuclear Cells from Amyotrophic Lateral Sclerosis Patients as a Potential Tool for Biomarker Research

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Author(s):
Araujo, Beatriz Grisolia [1] ; Souza e Silva, Luiz Felipe [1] ; Torresi, Jorge Luiz de Barros [1] ; Siena, Amanda [1] ; Valerio, Berenice Cataldo Oliveira [2] ; Brito, Mariana Dutra [1] ; Rosenstock, Tatiana Rosado [1]
Total Authors: 7
Affiliation:
[1] Santa Casa Sao Paulo Sch Med Sci, Dept Physiol Sci, Rua Doutor Cesario Motta Jr, 61 Vila Buarque, BR-01221020 Sao Paulo, SP - Brazil
[2] Irmandade Santa Casa Misericordia Sao Paulo, Dept Neurol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecular Neurobiology; AUG 2020.
Web of Science Citations: 0
Abstract

Amyotrophic lateral sclerosis (ALS) is a multifactorial and progressive neurodegenerative disease of unknown etiology. Due to ALS's unpredictable onset and progression rate, the search for biomarkers that allow the detection and tracking of its development and therapeutic efficacy would be of significant medical value. Considering that alterations of energy supply are one of ALS's main hallmarks and that a correlation has been established between gene expression in human brain tissue and peripheral blood mononuclear cells (PBMCs), the present work investigates whether changes in mitochondrial function could be used to monitor ALS. To achieve this goal, PBMCs from ALS patients and control subjects were used; blood sampling is a quite non-invasive method and is cost-effective. Different parameters were evaluated, namely cytosolic calcium levels, mitochondrial membrane potential, oxidative stress, and metabolic compounds levels, as well as mitochondrial dynamics and degradation. Altogether, we observed lower mitochondrial calcium uptake/retention, mitochondria depolarization, and redox homeostasis deregulation, in addition to a decrease in critical metabolic genes, a diminishment in mitochondrial biogenesis, and an augmentation in mitochondrial fission and autophagy-related gene expression. All of these changes can contribute to the decreased ATP and pyruvate levels observed in ALS PBMCs. Our data indicate that PBMCs from ALS patients show a significant mitochondrial dysfunction, resembling several findings from ALS' neural cells/models, which could be exploited as a powerful tool in ALS research. Our findings can also guide future studies on new pharmacological interventions for ALS since assessments of brain samples are challenging and represent a relevant limited strategy. (AU)

FAPESP's process: 15/02041-1 - The role of lysine(K)-deacetylases on mitochondrial disorders's neuroprotection: perspectives of epigenetic therapy for amyotrophic lateral sclerosis and schizophrenia
Grantee:Tatiana Rosado Rosenstock
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/09084-6 - Functional studies in lymphocytes from Amyotrophic Lateral Sclerosis patients: relevance to biomarkers determination
Grantee:Beatriz Grisolia Araujo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/25595-2 - Sirtuins in the neuropathology of schizophrenia: the role against mitochondrial dysfunction during hypoxia
Grantee:Luiz Felipe Souza e Silva
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/12039-7 - Lysine(K)-deacetylases modulation and its role in mitochondrial metabolism, dynamics and degradation: possible neuroprotection for amyotrophic lateral sclerosis
Grantee:Mariana Dutra Brito
Support type: Scholarships in Brazil - Master