Virulence factors and antimicrobial resistance inStaphylococcus aureusisolated from bovine mastitis in Brazil

Author(s)

Perez, Veronica K. C. ^[1] ; Custodio, Dirceia A. C. ^[1] ; Silva, Eduarda M. M. ^[1] ; de Oliveira, Julia ^[1] ; Guimaraes, Alessandro S. ^[2] ; Brito, Maria A. V. P. ^[2] ; Souza-Filho, Antonio F. ^[3] ; Heinemann, Marcos B. ^[3] ; Lage, Andrey P. ^[4] ; Dorneles, Elaine M. S. ^[1]

Affiliation

^[1] Univ Fed Lavras, Dept Med Vet, BR-37200900 Lavras, MG - Brazil

^[2] Embrapa Gado Leite, Empresa Brasileira Pesquisa Agr, BR-36038330 Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508900 Sao Paulo, SP - Brazil

^[4] Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270901 Belo Horizonte, MG - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 51, n. 4 AUG 2020.
• Web of Science Citations: 1

Abstract

This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400Staphylococcus aureusstrains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm geneicaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla(+)hlb(+)), 16.5% (hla(+)) and 0.75% (hlb(+)) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genesluk,seb, sec,sed, andtstwere observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The geneblaZwas detected in 82.03% of penicillin-resistant isolates, whereastetKandaac(6 `)-Ie-aph(2 `)-Iawere observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance genemepAwas detected for the first time inS. aureusfrom bovine mastitis. Resistance genestetM(3.22%), tetL(1.61%), ermA(14.29%), ermB(14.29%), ermC(33.3%), ermT(9.52%), ermY(4.76%), msrA(9.52%), andmphC(9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobial-resistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed thatS. aureusstrains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilm-forming and hemolytic, whereas virulence genes associated with enterotoxins, lukandtst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential ofS. aureusassociated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. (AU)

• FAPESP's process: 15/10332-6 - Study of the molecular diversity of strains of Staphylococcus aureus isolated from bovine mastitis
• Grantee: Marcos Bryan Heinemann
• Support Opportunities: Regular Research Grants