Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition

Author(s)

Sabo, Sabrina da Silva ^[1] ; Mendes, Maria Anita ^[2] ; Araujo, Elias da Silva ^[3] ; de Almeida Muradian, Ligia Bicudo ^[3] ; Makiyama, Edson Naoto ^[4] ; Guy LeBlanc, Jean ^[5] ; Borelli, Primavera ^[4] ; Fock, Ricardo Ambrosio ^[4] ; Knobl, Terezinha ^{[6, 7]} ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Chem Engn Dept, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[5] CERELA CONICET, San Miguel De Tucuman - Argentina

^[6] Univ Sao Paulo, Sao Paulo - Brazil

^[7] Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 APR 29 2020.
• Web of Science Citations: 0

Abstract

The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals' performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efficient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic effect towards many pathogens. Their capacity of producing the B-complex vitamins folate and riboflavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identified as Enterococcus faecium, while C173 and C195 were both identified as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/mL, respectively) and riboflavin (223.3 and 175.0ng/mL, respectively). (AU)

• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/16494-3 - Bioprospecting of probiotic bacteria producing bacteriocins and vitamins: an application as a feed additive in poultry for human consumption
• Grantee: Sabrina da Silva Sabo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral