Development of brushite particles synthesized in the presence of acidic monomers for dental applications

Author(s)

Chiari, Marina D. S. ^[1] ; Rodrigues, Marcela C. ^[2] ; Pinto, Mirella F. C. ^[1] ; Vieira, Douglas N. ^[1] ; Vichi, Flavio M. ^[3] ; Vega, Oscar ^[4] ; Chrzanowski, Wojciech ^[5] ; Nagaoka, Noriyuki ^[6] ; Braga, Roberto R. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Av Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

^[2] Cruzeiro do Sul Univ, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, Sao Paulo - Brazil

^[4] Nucl & Energy Res Inst, Ctr Chem & Environm Technol, Sao Paulo - Brazil

^[5] Univ Sydney, Sch Pharm, Sydney, NSW - Australia

^[6] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Okayama, Okayama - Japan

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 116, NOV 2020.
• Web of Science Citations: 0

Abstract

Objectives: To synthesize and characterize brushite particles in the presence of acidic monomers (acrylic acid/AA, citric acid/CA, and methacryloyloxyethyl phosphate/MOEP) and evaluate the effect of these particles on degree of conversion (DC), flexural strength/modulus (FS/FM) and ion release of experimental composites. Methods: Particles were synthesized by co-precipitation with monomers added to the phosphate precursor solution and characterized for monomer content, size and morphology. Composites containing 20 vol% brushite and 40 vol% reinforcing glass were tested for DC, FS and FM (after 24 h and 60 d in water), and 60-day ion release. Data were subjected to ANOVA/Tukey tests (DC) or Kruskal-Wallis/Dunn tests (FS and FM, alpha: 5%). Results: The presence of acidic monomers affected particle morphology. Monomer content on the particles was low (0.1-1.4% by mass). Composites presented similar DC. For FS/24 h, only the composite containing DCPD\_AA was statistically similar to the composite containing 60 vol% of reinforcing glass (without brushite, ``control{''}). After 60 days, all brushite-containing materials showed similar FS, statistically lower than the control composite (p < 0.01). Composites containing DCPD\_AA, DCPD\_MOEP or DCPD\_U ({''}unmodified{''}) showed statistically similar FM/24 h, higher than the control composite. After prolonged immersion, all composites were similar to the control composite, except DCPD\_AA. Cumulative ion release ranged from 21 ppm to 28 ppm (calcium) and 9 ppm to 17 ppm (phosphate). Statistically significant reductions in ion release between 15 and 60 days were detected only for the composite containing DCPD\_MOEP. Significance: Acidic monomers added to the synthesis affected brushite particle morphology. After 60-day storage in water, composite strength was similar among all brushite-containing composites. Ion release was sustained for 60 days and it was not affected by particle morphology. (AU)

• FAPESP's process: 17/06446-1 - Effect of calcium orthophosphate composites on microhardness of enamel exposed to cariogenic challenge
• Grantee: Mirella Flávia Corrêa Pinto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/50321-6 - Development of calcium phosphate nanoparticles functionalized with acidic monomers for dental remineralization
• Grantee: Roberto Ruggiero Braga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/25971-7 - Development and application of calcium phosphate nanoparticles functionalized with acidic monomers for dental remineralization
• Grantee: Marina Damasceno e Souza de Carvalho Chiari
• Support Opportunities: Scholarships in Brazil - Master