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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network

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Danielle Bastos Araujo ; Rafael Rahal Guaragna Machado ; Deyvid Emanuel Amgarten ; Fernanda de Mello Malta ; Gabriel Guarany de Araujo ; Cairo Oliveira Monteiro ; Erika Donizetti Candido ; Camila Pereira Soares ; Fernando Gatti de Menezes ; Ana Carolina Cornachioni Pires ; Rúbia Anita Ferraz Santana ; Amanda de Oliveira Viana ; Erick Dorlass ; Luciano Thomazelli ; Luis Carlos de Sousa Ferreira ; Viviane Fongaro Botosso ; Cristiane Rodrigues Guzzo Carvalho ; Danielle Bruna Leal Oliveira ; João Renato Rebello Pinho ; Edison Luiz Durigon
Total Authors: 20
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 115, p. -, 2020.
Abstract

BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research. (AU)

FAPESP's process: 17/25123-9 - Monitoring of ectoparasites and viruses in bats in urban Atlantic Forest fragment
Grantee:Amanda de Oliveira Viana
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/21076-9 - Structure of the Xanthomonas citri type IV pilus secretin
Grantee:Gabriel Guarany de Araujo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/23680-0 - Standardization of Sherlock technique (Specific High-Sensitivity Enzymatic Reporter Unlocking) using the Cas13a protein for the diagnosis of respiratory syncytial virus in pediatric patients
Grantee:Camila Pereira Soares
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/24769-2 - Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization
Grantee:Rafael Rahal Guaragna Machado
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 20/04680-0 - Design of different fragments of SARS-CoV-2 surface protein spike for the development of fast diagnostic test and vaccine
Grantee:Cristiane Rodrigues Guzzo Carvalho
Support type: Regular Research Grants
FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
Grantee:Edison Luiz Durigon
Support type: Regular Research Grants
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants