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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serum folate and cytokines in heterozygous beta-thalassemia

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Paniz, Clovis [1, 2] ; Lucena, Maylla Rodrigues [3] ; Bertinato, Juliano Felix [1] ; dos Santos, Magnun Nueldo Nunes [4] ; Gomes, Guilherme Wataru [1] ; Figueiredo, Maria Stella [3] ; Sonati, Maria de Fatima [4] ; Blaia-D Avila, Vera Lucia Nascimento [5] ; Green, Ralph [6] ; Guerra-Shinohara, Elvira Maria [1, 3, 7]
Total Authors: 10
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[2] Univ Fed Santa Maria, Dept Anal Clin & Toxicol, Santa Maria, RS - Brazil
[3] Univ Fed Sao Paulo, Disciplina Hematol & Hemoterapia, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, Sao Paulo - Brazil
[5] Pontificia Univ Catolica Sao Paulo, Fac Med & Ciencias Saude, Div Hematol, Sorocaba - Brazil
[6] UC Davis Sch Med, Dept Pathol & Lab Med, Sacramento, CA - USA
[7] Univ Fed Mato Grosso do Sul, Fac & Ciencias Farmaceut Alimentos & Nutr, Campo Grande, MS - Brazil
Total Affiliations: 7
Document type: Journal article
Web of Science Citations: 0

Introduction Folate deficiency is commonly reported in beta-thalassemia. Individuals heterozygous for beta-thalassemia may have higher folate requirements than normal individuals. Objectives: To document the concentration of serum total folate and its forms in beta-thalassemia heterozygote users (beta-TmU) and nonusers (beta-TmN) of 5 mg folic acid/d; to determine whether folic acid (FA) consumption from fortified foods allows beta-Tm patients, who do not take FA supplements, to meet their dietary folate requirements; and to investigate the association between higher serum unmetabolized folic acid (UMFA) and inflammatory cytokine concentrations. Methods Serum total folate and forms were measured in 42 beta-Tm (13 beta-TmU and 29 beta-TmN) and 84 healthy controls. The mononuclear leucocyte mRNA expression of relevant genes and their products and hematological profiles were determined. Results beta-TmU had higher serum total folate, 5-methyltetrahydrofolate, UMFA, and tetrahydrofolate (THF) compared with beta-TmN. The beta-TmN had lower serum total folate and THF than controls. Plasma total homocysteine (tHcy) was lower in beta-TmU compared with both beta-TmN and controls, while beta-TmN had higher tHcy than controls. beta-TmU had higher IL-8 than their controls while beta-TmN had higher IL-6 and IL-8 than their controls. beta-TmU have higher levels of serum total folate, 5- methyltetrahydrofolate, UMFA, and THF than controls. There was no association between UMFA concentrations and cytokine levels. Conclusions Mandatory flour fortification with FA in Brazil may be insufficient for beta-TmN, since they have higher tHcy and lower serum total folate than controls. Furthermore, beta-TmN have higher IL-6 levels than beta-TmU. UMFA was not associated with inflammatory cytokine levels. (AU)

FAPESP's process: 12/12912-1 - Effects of unmetabolized folic acid on global DNA methylation, mRNA expression and NK cells cytotoxicity in individuals exposed to mandatory fortification with folic acid and / or therapeutic use of this vitamin
Grantee:Elvira Maria Guerra Shinohara
Support type: Regular Research Grants