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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Invariant Natural Killer T cells resilience to paradoxical sleep deprivation-associated stress

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Sousa, Maria E. P. [1] ; Gonzatti, Michelangelo B. [1] ; Fernandes, Edgar R. [1] ; Freire, Beatriz M. [1] ; Guereschi, Marcia G. [1] ; Basso, Alexandre S. [1] ; Andersen, Monica L. [2] ; Rosa, Daniela S. [3, 1] ; Keller, Alexandre C. [1]
Total Authors: 9
[1] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Rua Botucatu, 4th Floor, BR-04023062 Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP EPM, Dept Psychobiol, Rua Botucatu, 862, 1st Floor, BR-04023062 Sao Paulo - Brazil
[3] Inst Invest Immunol Iii INCT, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BRAIN BEHAVIOR AND IMMUNITY; v. 90, p. 208-215, NOV 2020.
Web of Science Citations: 0

Although several studies demonstrate that stressful situations, such as sleep disturbances, negatively impact the innate and adaptive arms of the immune system, their influence on invariant Natural Killer T (iNKT) cells remains unclear. iNKT cells are CD1d-restricted innate T cells that recognize glycolipid antigens and rapidly produce polarizing cytokines being key players in several immune responses, and a potential target for immunotherapy. iNKT cells differ in several aspects from conventional T lymphocytes, including a unique dependence on CD1d-expressing double-positive (DP) thymocytes for intrathymic maturation. As a consequence of stress, DP thymocytes undergo glucocorticoid-induced apoptosis, which might compromise iNKT developmental pathway. Therefore, we used a paradoxical sleep deprivation (SD) model to determine the impact of sleep disturbance on iNKT cell biology. After 72 h of SD, C57Bl/6 mice exhibited a significant increase in systemic glucocorticoid levels and thymus atrophy. Despite marked decrease in the number of DP thymocytes, the ratio CD1d(+)/CD1d(-) was higher in SD mice, and the number of thymic iNKT cells remained unaltered, suggesting that SD did not compromise the iNKT developmental pathway. In contrast, SD reduced hepatic IFN-gamma, but not, IL-4-producing iNKT cells, without further effect in the spleen. Despite this fact, SD did not affect stimulation of IFN-gamma production by iNKT cells, or cytokine release, in response to alpha-galactosylceramide, a specific antigen. Furthermore, although SD impaired splenic NK cells activity against tumor cells, it did not affect iNKT cell-specific cytotoxicity. Thus, our study shows that SD-induced stress did not impair the iNKT cells' responses to a cognate antigen. (AU)

FAPESP's process: 17/17471-7 - Antigenicity and immunogenicity of Zika virus envelope recombinant proteins
Grantee:Daniela Santoro Rosa
Support type: Regular Research Grants
FAPESP's process: 19/11490-5 - The role of the sympathetic nervous system on the biological activity of invariant Natural Killer T lymphocytes
Grantee:Alexandre de Castro Keller
Support type: Regular Research Grants