| Full text | |
| Author(s): |
Reis, Natasha Ferraz de Campos
[1]
;
Dupin, Talita Vieira
[1]
;
Costa, Carolina Rizzaro
[1]
;
Toledo, Mayte dos Santos
[1]
;
de Oliveira, Vivian Cristina
[2]
;
Popi, Ana Flavia
[2]
;
Torrecilhas, Ana Claudia
[1]
;
Xander, Patricia
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Pharmaceut Sci, Lab Cellular Immunol & Biochem Fungi & Protozoa, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Paulista Sch Med, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, OCT 30 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
B-1 cells are considered an innate-like B cell population that participates in effective innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory sites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and participate in immunity against Leishmania. Our group showed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental infection by L. amazonensis. However, the B-1 cells' responses to Leishmania or EVs isolated from parasites are still poorly characterized. Studying the activation and differentiation of B-1 cells in vivo can contribute to a better understanding of how these cells participate in immunity to L. amazonensis. Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage commitment factors, Toll-like receptors (TLRs), activation cell surface markers, nitric oxide (NO) and reactive oxygen species (ROS) production in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs released by the parasites. Our results demonstrated that L. amazonensis infection did not stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, but a significant decrease in the production of NO and ROS was observed. The infection induced a significantly higher arginase expression in B-1 cells, but the stimulation with EVs led to a decrease in this gene expression. TLR-2 and TLR-6 had significantly higher expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was higher in animals infected or stimulated for 48 h with EVs. Interestingly, in B-1 cells the stimulus with L. amazonensis led to a substantial increase in the expression of myeloid restricted transcription factors. Thus, our study suggests that the parasites or EVs differently modulated the activation and differentiation of B-1 cells. (AU) | |
| FAPESP's process: | 19/21614-3 - Extracellular vesicles released by Leishmania (Leishmania) amazonensis with distinct virulence profiles: characterization, role in immune response and disease progression |
| Grantee: | Patricia Xander Batista |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/06597-2 - Study of the effects of extracellular vesicles released by Leishmania amazonensis promastigotes on macrophages response and disease progression |
| Grantee: | Natasha Ferraz de Campos Reis |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 16/17245-4 - Study of the effects of extracellular vesicles released by Leishmania amazonensis promastigotes on macrophages response and disease progression |
| Grantee: | Patricia Xander Batista |
| Support Opportunities: | Regular Research Grants |