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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A

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Author(s):
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Morao, Luana G. [1] ; Lorenzoni, Andre S. G. [2] ; Chakraborty, Parichita [2] ; Ayusso, Gabriela M. [3] ; Cavalca, Lucia B. [2] ; Santos, Mariana B. [3] ; Marques, Beatriz C. [3] ; Dilarri, Guilherme [1] ; Zamuner, Caio [1] ; Regasini, Luis O. [3] ; Ferreira, Henrique [1] ; Scheffers, Dirk-Jan [2]
Total Authors: 12
Affiliation:
[1] Univ Estadual Paulista, Dept Bioquim & Microbiol, Inst Biociencias, BR-13050690 Rio Claro, SP - Brazil
[2] Univ Groningen, Dept Mol Microbiol, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen - Netherlands
[3] Univ Estadual Paulista, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 25, n. 20 OCT 2020.
Web of Science Citations: 0
Abstract

Xanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 mu g/mL to 40 mu g/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action. (AU)

FAPESP's process: 16/07108-0 - Preparation and Biological Evaluation of Curcumin-Cinnamaldehyde Hybrids as Bacterial Cell Division Inhibitors Dehydrozingerone Derivatives (Part II)
Grantee:Gabriela Miranda Ayusso
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/50216-0 - Using agricultural waste to combat plant pathogens environmental friendly ways to combat Xanthomonas citri
Grantee:Henrique Ferreira
Support type: Regular Research Grants
FAPESP's process: 14/18577-5 - Preparation and Biological Evaluation of Curcumin-Cinnamaldehyde Hybrids as Bacterial Cell Division Inhibitors: Methoxychalcones
Grantee:Beatriz de Carvalho Marques
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/18330-0 - Synthesis and biological evaluation of curcumin-cinnamaldehyde hybrids as bacterial cell division inhibitors
Grantee:Luis Octávio Regasini
Support type: Regular Research Grants
FAPESP's process: 18/15083-2 - Synthesis and Biological Evaluation of Isobavachalcone (IBC) and Analogs as Potential Agents against Tuberculosis
Grantee:Luis Octávio Regasini
Support type: Regular Research Grants
FAPESP's process: 16/08084-7 - Preparation and Biological Evaluation of Curcumin-Cinnamaldehyde Hybrids as Bacterial Cell Division Inhibitors: Methoxychalcones (Part 2)
Grantee:Beatriz de Carvalho Marques
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/18719-4 - Preparation and Biological Evaluation of Curcumin-Cinnamaldehyde Hybrids as Bacterial Cell Division Inhibitors: Dehydrozyngerone Derivatives
Grantee:Gabriela Miranda Ayusso
Support type: Scholarships in Brazil - Scientific Initiation