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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dengue RNA detection and seroprevalence in blood donors during an outbreak in the Sao Paulo State, Brazil, 2016

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Slavov, Svetoslav N. [1, 2] ; Santos, V, Elaine ; Hespanhol, Marta R. [3] ; Rodrigues, Evandra S. [3] ; Haddad, Rodrigo [4, 5] ; Ubiali, Eugenia M. A. [3] ; Covas, Dimas T. [1, 3] ; Kashima, Simone [3]
Total Authors: 8
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Santos, Elaine, V, Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Brasilia, Fac Ceilandia, Lab Clin Anal, Brasilia, DF - Brazil
[5] Univ Brasilia, Fac Med, Ctr Trop Med, Brasilia, DF - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Medical Virology; v. 93, n. 6 OCT 2020.
Web of Science Citations: 0

Most dengue virus (DENV) infections remain asymptomatic. This increases the risk of DENV transfusion transmission (TT-DENV) during outbreaks. We evaluated DENV viremia in 8475 blood donations assembled in minipools for the presence of DENV RNA. The tested samples were obtained between February and May, 2016, during a large DENV outbreak in Ribeirao Preto city, northeast region of the Sao Paulo State, Brazil. The DENV RNA + samples were serotyped and screened for DENV NS1. We also tested a significant number of plasma samples (n = 372) to estimate the DENV seroprevalence among blood donors in the region. We detected three DENV RNA + samples in the tested blood donations (n = 3/8475, 0.04%). From these, two samples were further serotyped as DENV-1 and one sample as DENV-2. All DENV RNA positive samples were negative for anti-DENV IgG, indicating the presence of primary acute infection. Moreover, two of the DENV RNA + samples were also NS1 antigen positive (antigenemia). The anti-DENV IgG seroprevalence among blood donor population was 50.8% (n = 189/372). Our results are in accordance with the presence of DENV primary infection in blood donors which can lead to transfusion transmission of the infection to recipients. Measures to exclude such donors should be adopted to prevent TT-DENV. (AU)

FAPESP's process: 14/50947-7 - INCT 2014: in Stem Cell and Cell Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/15049-3 - Development and validation of multiplex molecular platform for confirmatry diagnosis of Zika virus infection and establishment of laboratory standards
Grantee:Simone Kashima Haddad
Support type: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)