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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recovering the osteoblastic differentiation potential of mesenchymal stem cells derived from diabetic rats by photobiomodulation therapy

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Author(s):
Bueno, Natalia Pieretti [1] ; Copete, Isabella Nunes [1] ; Lopes, Helena Bacha [2] ; Arany, Praveen R. [3] ; Marques, Marcia Martins [1, 4] ; Ferraz, Emanuela Prado [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Sao Paulo - Brazil
[3] SUNY Buffalo, Sch Dent Med, New York, NY - USA
[4] Univ Ibirapuera, Sch Dent, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Biophotonics; v. 14, n. 3 NOV 2020.
Web of Science Citations: 0
Abstract

Autologous cell-based therapy for bone regeneration might be impaired by diabetes mellitus (DM) due to the negative effects on mesenchymal stem cells (MSCs) differentiation. Strategies to recover their osteogenic potential could optimize the results. We aimed to evaluate the effect of photobiomodulation (PBM) therapy on osteoblast differentiation of rats with induced DM. Bone marrow MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB, respectively). dOB were treated with PBM therapy every 72 hour (660 nm; 0.14 J; 20 mW; 0.714 W/cm(2), and 5 J/cm(2)). Cell morphology, viability, gene and protein expression of osteoblastic markers, alkaline phosphatase (ALP) activity, and the mineralized matrix production of dOB-PBM were compared to dOB. PBM therapy improved viability of dOB, increased the gene and protein expression of bone markers, the ALP activity and the mineralized matrix production. PBM therapy represents an innovative therapeutic approach to optimize the treatment of bone defects in diabetic patients. (AU)

FAPESP's process: 18/20302-5 - The effect of photobiomodulation therapy on osteoblast differentiation of mesenchymal stem cell derived from bone marrow of diabetic rats
Grantee:Isabella Nunes Copete
Support type: Scholarships in Brazil - Scientific Initiation