Comparative proteomic analysis of dental cementum from deciduous and permanent teeth

Author(s)

Giovani, Priscila A. ^[1] ; Martins, Luciane ^[2] ; Salmon, Cristiane R. ^[2] ; Mofatto, Luciana S. ^[3] ; Leme, Adriana F. P. ^[4] ; Puppin-Rontani, Regina M. ^[1] ; Kolli, Tamara N. ^[5] ; Foster, Brian L. ^[5] ; Nociti, Jr., Francisco H. ^[2] ; Kantovitz, Kamila R. ^{[6, 1]}

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Div Periodont, Dept Prosthodont & Periodont, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil

^[4] CNPEM, LNBio, Brazilian Biosci Natl Lab, Campinas, SP - Brazil

^[5] Ohio State Univ, Coll Dent, Div Biosci, Columbus, OH 43210 - USA

^[6] Sao Leopoldo Mand Res Ctr, Dept Dent Mat, Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PERIODONTAL RESEARCH; v. 56, n. 1 NOV 2020.
• Web of Science Citations: 0

Abstract

Background and Objectives Dental cementum (DC) is a mineralized tissue covering tooth roots that plays a critical role in dental attachment. Differences in deciduous vs. permanent tooth DC have not been explored. We hypothesized that proteomic analysis of DC matrix would identify compositional differences in deciduous (DecDC) vs. permanent (PermDC) cementum that might reflect physiological or pathological differences, such as root resorption that is physiological in deciduous teeth but can be pathological in the permanent dentition. Methods Protein extracts from deciduous (n = 25) and permanent (n = 12) teeth were pooled (five pools of DecDC, five teeth each; four pools of PermDC, three teeth each). Samples were denatured, and proteins were extracted, reduced, alkylated, digested, and analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). The beta-binomial statistical test was applied to normalized spectrum counts with 5% significance level to determine differentially expressed proteins. Immunohistochemistry was used to validate selected proteins. Results A total of 510 proteins were identified: 123 (24.1%) exclusive to DecDC; 128 (25.1%) exclusive to PermDC; 259 (50.8%) commonly expressed in both DecDC and PermDC. Out of 60 differentially expressed proteins, 17 (28.3%) were detected in DecDC, including myeloperoxidase (MPO), whereas 43 (71.7%) were detected in PermDC, including decorin (DCN) and osteocalcin (BGLAP). Overall, Gene Ontology (GO) analysis indicated that all expressed proteins were related to GO biological processes that included localization and response to stress, and the GO molecular function of differentially expressed proteins was enriched in cell adhesion, molecular binding, cytoskeletal protein binding, structural molecular activity, and macromolecular complex binding. Immunohistochemistry confirmed the trends for selected differentially expressed proteins in human teeth. Conclusions Clear differences were found between the proteomes of DecDC and PermDC. These findings may lead to new insights into developmental differences between DecDC and PermDC, as well as to a better understanding of physiological/pathological events such as root resorption. (AU)

• FAPESP's process: 16/02942-1 - Global Proteome Profile of Periodontal Ligament.
• Grantee: Priscila Alves Giovani
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
• Grantee: Munir Salomao Skaf
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/13786-0 - Effect of Novel Nanotechnology to Conventional Glass Ionomer Cement on its Physical-Chemical-Biological Properties
• Grantee: Kamila Rosamilia Kantovitz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/13242-9 - Comparative proteomic analysis of dental cementum from deciduous and permanent teeth
• Grantee: Priscila Alves Giovani
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate