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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Lung Microbiome of Three Young Brazilian Patients With Cystic Fibrosis Colonized by Fungi

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Author(s):
de Almeida, Otavio Guilherme Goncalves [1] ; Capizzani, Carolina Paulino da Costa [1] ; Tonani, Ludmilla [1] ; Grizante Bariao, Patricia Helena [1] ; da Cunha, Anderson Ferreira [2] ; De Martinis, Elaine Cristina Pereira [1] ; Torres, Lidia Alice Gomes Monteiro Marin [3] ; von Zeska Kress, Marcia Regina [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Ctr Ciencias Biol & Saude, Sao Carlos - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Hosp Clin, Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, NOV 11 2020.
Web of Science Citations: 0
Abstract

Microbial communities infiltrate the respiratory tract of cystic fibrosis patients, where chronic colonization and infection lead to clinical decline. This report aims to provide an overview of the diversity of bacterial and fungal species from the airway secretion of three young CF patients with severe pulmonary disease. The bacterial and fungal microbiomes were investigated by culture isolation, metataxonomics, and metagenomics shotgun. Virulence factors and antibiotic resistance genes were also explored. A. fumigatus was isolated from cultures and identified in high incidence from patient sputum samples. Candida albicans, Penicillium sp., Hanseniaspora sp., Torulaspora delbrueckii, and Talaromyces amestolkiae were isolated sporadically. Metataxonomics and metagenomics detected fungal reads (Saccharomyces cerevisiae, A. fumigatus, and Schizophyllum sp.) in one sputum sample. The main pathogenic bacteria identified were Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia cepacia complex, and Achromobacter xylosoxidans. The canonical core CF microbiome is composed of species from the genera Streptococcus, Neisseria, Rothia, Prevotella, and Haemophilus. Thus, the airways of the three young CF patients presented dominant bacterial genera and interindividual variability in microbial community composition and diversity. Additionally, a wide diversity of virulence factors and antibiotic resistance genes were identified in the CF lung microbiomes, which may be linked to the clinical condition of the CF patients. Understanding the microbial community is crucial to improve therapy because it may have the opposite effect, restructuring the pathogenic microbiota. Future studies focusing on the influence of fungi on bacterial diversity and microbial interactions in CF microbiomes will be welcome to fulfill this huge gap of fungal influence on CF physiopathology. (AU)

FAPESP's process: 17/13759-6 - Metagenomic prospection of quorum sensing related bacteria during spontaneous cocoa beans fermentation
Grantee:Otávio Guilherme Gonçalves de Almeida
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/25300-8 - Study of fungal clinical isolates from different anatomical sites and metagenomic of the lung microbiome of patients with cystic fibrosis
Grantee:Marcia Regina von Zeska Kress
Support Opportunities: Regular Research Grants