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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Assessment of the Bioactive Potential of Cheese Whey Protein Hydrolysates Using Immobilized Alcalase

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da Cruz, Clariana Zanutto Paulino [1] ; de Mendonca, Ricardo Jose [2] ; Guimaraes, Luis Henrique Souza [3] ; dos Santos Ramos, Matheus Aparecido [4] ; Garrido, Saulo Santesso [1] ; de Paula, Ariela Veloso [4] ; Monti, Rubens [4] ; Massolini, Gabriella [5]
Total Authors: 8
[1] Sao Paulo State Univ UNESP, Chem Inst, BR-14800060 Araraquara, SP - Brazil
[2] Fed Univ Triangulo Mineiro UFTM, Biochem Sect, BR-38015050 Uberaba, MG - Brazil
[3] Sao Paulo Univ USP, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil
[5] Univ Pavia, Dipartimento Sci Farm, I-27100 Pavia, PV - Italy
Total Affiliations: 5
Document type: Journal article
Source: Food and Bioprocess Technology; v. 13, n. 12 NOV 2020.
Web of Science Citations: 0

The hydrolysis of bovine cheese whey (BCW) proteins was performed by the Alcalase immobilized on the support glyoxyl-corn-cob-powder (AGCCP). The whey protein hydrolysates (WPH) obtained with AGCPP were fractionated by RP-HPLC according to the hydrophilicity of the peptides (F1, hydrophilic; F2, intermediate hydrophilicity; and F3, hydrophobic). The fractions were analyzed by MALDI-TOF, and molecular weight peptides (< 1500 m/z) were identified. Total WPH obtained by AGCCP presented high capacity reduction of the ABTS radical (57.82%) and high chelating activity of iron II (76.2%). The obtained peptides showed high antimicrobial activity (87.75-100%) against the species Escherichia coli (ATCC 43895) and Listeria monocytogenes (ATCC 7644). Only F2 was effective against the Candida albicans strain ATCC 18804 tested (MIC = 10 mg/mL). The results of this study show that the hydrolysis of BCW proteins using the AGCCP derivative represents a very interesting and viable way for obtention of bioactive peptides from cheese whey. (AU)

FAPESP's process: 12/07680-4 - Partial hydrolysates of whey proteins from bovine and buffalo milk obtained with immobilised proteases: peptides transportation and absorption through dialyzability and Caco-2 cell culture
Grantee:Juliana Cristina Bassan
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/12563-2 - Agro industrial residues as solid support for immobilization and stabilization of enzymes of biotechnological interest
Grantee:Rubens Monti
Support type: Regular Research Grants