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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Situ Proteomic Analysis of Glioblastoma Multiforme: A Translational Approach to Improve Prognostic/Diagnostic Routines

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Author(s):
da Silva Menegasso, AnaIly Ribeiro [1] ; Pratavieira, Marcel [1] ; dos Santos, Lucilene Delazari [2] ; Lima, Flavio de Oliveira [2] ; Moraes, Marcelo Padovani T. [3] ; Zanini, Marco Antonio [3] ; Palma, Mario Sergio [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ UNESP, Ctr Study Social Insects, Dept Basic & Appl Biol, Inst Biosci, Rio Claro, SP - Brazil
[2] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 7, n. 29, p. 64-83, OCT-DEC 2020.
Web of Science Citations: 0
Abstract

In order to evaluate the use of matrix-assisted laser desorption ionization (MALDI) Mass Spectral Imaging (MSI) to Glioblastoma (GBM) studies, some sections of formalin fixed paraffin embedded samples of GBM tumors were submitted to classical immunoassays, to define the profile of distribution of some classical and well recognized molecular markers of GBM grade IV (Ki-67, S100, Glial GFAP, CD31 and CD34), while other sections of the same samples were submitted to in-tissueproteomic analysis by MALDI MSI, and both results compared to each other. The overlapping of the MALDI spectra obtained for the tryptic peptides with the immunohistochemical reactions of each marker protein were used to build a distribution map of the marker proteins all over the GBM tissue section. The results revealed a high correlation between both methods, indicating that MALDI MSI has enough sensitivity to be compared to the immunohistochemical methods, as well is sufficiently reliable to be used in biomarkers identification. (AU)

FAPESP's process: 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants