Acquired pellicle protein-based engineering protects against erosive demineralization

Author(s):Show less -

Carvalho, Thamyris Souza ^[1] ; Araujo, Tamara Teodoro ^[1] ; Oliveira Ventura, Talita Mendes ^[1] ; Dionizio, Aline ^[1] ; Frazao Camara, Joao Victor ^[1] ; Moraes, Samanta Mascarenhas ^[1] ; Pela, Vinicius Taioqui ^[1] ; Martini, Tatiana ^[1] ; Leme, Julia Chaparro ^[1] ; Bogaz Derbotolli, Ana Luiza ^[1] ; Grizzo, Larissa Tercilia ^[1] ; Crusca, Edson ^[2] ; Tanaka Shibao, Priscila Yumi ^[3] ; Marchetto, Reinaldo ^[2] ; Henrique-Silva, Flavio ^[3] ; Pessan, Juliano Pelim ^[4] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Technol, Araraquara, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Prevent & Restorat Dent, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 102, NOV 2020.
• Web of Science Citations: 0

Abstract

Objectives: To evaluate, in vivo: 1) proteomic alterations in the acquired enamel pellicle (AEP) after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), statherin-derived peptide (StN15) or their combination before the formation of the AEP and subsequent erosive challenge; 2) the protection of these treatments against erosive demnineralization. Materials and methods: In 5 crossover phases, after prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with: deionized water-1, 0.1 mg/mL CaneCPI-5-2, 1.0 mg/mL HB-3, 1.88 x 10(-5) M StN15-4 or their combination-5. AEP was formed (2 h) and enamel biopsy (10 mu L, 1%citric acid, pH 2.5, 10 s) was performed on one incisor for calcium analysis. The same acid was applied on the vestibular surfaces of the remaining teeth. The acid-resistant proteins within the remaining AEP were collected. Samples were quantitatively analyzed by label-free proteomics. Results: Treatment with the proteins/peptide, isolated or combined, increased several acid-resistant proteins in the AEP, compared with control. The highest increases were seen for PRPs (32-fold, StN15), profilin (15-fold, combination), alpha-amylase (9-fold; StN15), keratins (8-fold, CaneCPI-5 and HB), Histatin-1 (7-fold, StN15), immunoglobulins (6.5-fold, StN15), lactotransferrin (4-fold, CaneCPI-5), cystatins, lysozyme, protein S-100-A9 and actins (3.5-fold, StN15), serum albumin (3.5-fold, CaneCPI-5 and HB) and hemoglobin (3-fold, StN15). Annexin, calmodulin, keratin, tubulin and cystatins were identified exclusively upon treatment with the proteins/peptide, alone or combined. Groups 2, 3 and 4 had significantly lower Ca released from enamel compared to group 1 (Kruskal-Wallis/Dunn's, p < 0.05). Conclusions: Treatment with CaneCPI-5, HB or StN15 remarkably increases acid-resistant proteins in the AEP, protecting against erosion. Clinical significance: Our results show, for the first time, that treatment with proteins/peptide remarkably increases acid-resistant proteins in the AEP, protecting against erosive demineralization. These findings open an avenue for a new preventive approach for erosive demineralization, employing acquired pellicle engineering procedures that may in the future be incorporated into dental products. (AU)

• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/16254-8 - Acquired enamel film engineering for protection against tooth erosion: in vivo evaluation of the protective effect of sugarcane-derived cystatin (CaneCPI-5), hemoglobin and/or peptide derived from staterin
• Grantee: Thamyris de Souza Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate