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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Extracellular biofilm matrix leads to microbial dysbiosis and reduces biofilm susceptibility to antimicrobials on titanium biomaterial: An in vitro and in situ study

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Author(s):
Costa, Raphael Cavalcante [1] ; Souza, Joao Gabriel Silva [1] ; Bertolini, Martinna [2] ; Retamal-Valdes, Belen [3] ; Feres, Magda [3] ; Barao, Valentim A. R. [1]
Total Authors: 6
Affiliation:
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Connecticut UCONN, Sch Dent Med, Farmington, CT - USA
[3] Guarulhos Univ UNG, Dent Res Div, Dept Periodontol, Guarulhos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Clinical Oral Implants Research; v. 31, n. 12, p. 1173-1186, DEC 2020.
Web of Science Citations: 0
Abstract

Objectives: To test the role of exopolysaccharide (EPS) polymers matrix to modulate the composition/virulence of biofilms growing on titanium (Ti) surfaces, the effect on antibiotic susceptibility, and whether a dual-targeting therapy approach for disrupted EPS matrix could improve the antimicrobial effect. Materials and Methods: A microcosm biofilm model using human saliva as inoculum was used, and the microbial composition was assessed by checkerboard DNA-DNA hybridization. EPS-enriched biofilms virulence was tested using fibroblast monolayer. Povidone-iodine (PI) was used as EPS-targeting agent followed by amoxicillin + metronidazole antibiotic to reduce bacterial biomass using an in situ model. Results: An EPS-enriched environment, obtained by sucrose exposure, promoted bacterial accumulation and led to a dysbiosis on biofilms, favoring the growth ofStreptococcus, Fusobacterium,andCampylobacterspecies and even strict anaerobic species related to peri-implant infections, such asPorphyromonas gingivalisandTannerella forsythia(similar to 3-fold increase). EPS-enriched biofilm transitioned from a commensal aerobic to a pathogenic anaerobic profile. EPS increased biofilm virulence promoting higher host cell damage and reduced antimicrobial susceptibility, but the use of a dual-targeting approach with PI pre-treatment disrupted EPS matrix scaffold, increasing antibiotic effect on in situ biofilms. Conclusion: Altogether, our data provide new insights of how EPS matrix creates an environment that favors putative pathogens growth and shed light to a promising approach that uses matrix disruption as initial step to potentially improve implant-related infections treatment. (AU)

FAPESP's process: 18/04630-2 - Synthesis of bioactive glass coating for titanium surface using the plasma electrolytic oxidation: electrochemical and antimicrobial analysis in a microcosm model
Grantee:Raphael Cavalcante Costa
Support Opportunities: Scholarships in Brazil - Master