| Full text | |
| Author(s): |
Rafaella Fabiana Carneiro Pereira
[1]
;
Thais Holtz Theizen
[2]
;
Daisy Machado
[3]
;
João Paulo de Oliveira Guarnieri
[4]
;
Gabriel Piccirillo Gomide
[5]
;
Luciana Maria de Hollanda
[6]
;
Marcelo Lancellotti
Total Authors: 7
|
| Affiliation: | [1] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica e Biologia Tecidual - Brasil
[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica e Biologia Tecidual - Brasil
[3] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica e Biologia Tecidual - Brasil
[4] Universidade Estadual de Campinas. Faculdade de Ciências Farmacêuticas - Brasil
[5] Universidade Estadual de Campinas. Faculdade de Ciências Farmacêuticas - Brasil
[6] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Bioquímica e Biologia Tecidual - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | GENETICS AND MOLECULAR BIOLOGY; v. 44, n. 1 2020-12-21. |
| Abstract | |
Abstract Brazilian Purpuric Fever (BPF) is a hemorrhagic pediatric illness caused by Haemophilus influenzae biogroup aegyptius (Hae), a bacterium that was formerly associated with self-limited purulent conjunctivitis. BPF is assumed to be eradicated. However, the virulence mechanisms inherent to Hae strains associated with BPF is still a mystery and deficient in studies. Here, we aim to analyze the role of the autotransporter genes related to adherence and colonization las, tabA1, and hadA genes through RT-qPCR expression profiling and knockout mutants. Relative quantification by real-time PCR after infection in human cells and infant rat model suggests that las was initially downregulated probably duo to immune evasion, tabA1, and hadA were overexpressed in general, suggesting an active role of TabA1 and HadA1 adhesins in Hae in vitro and in vivo. Transformation attempts were unsuccessful despite the use of multiple technical approaches and in silico analysis revealed that Hae lacks genes related to competence in Haemophilus, which could be part of the elucidation of the difficulty of genetically manipulating Hae strains. (AU) | |
| FAPESP's process: | 11/01319-5 - Study of the genome and virulence of Haemophilus influenzae biotype aegyptius associated with Brazilian Purpuric Fever |
| Grantee: | Rafaella Fabiana Carneiro Pereira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/09874-7 - Analysis and use of the genome of Haemophilus influenzae biotype aegyptius for biotechnologies purposes |
| Grantee: | Marcelo Lancellotti |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/00847-3 - Evaluation of Barbatimão e Aroeira mesoporous sílica nanoparticle in human keratinocyte (HaCaT) and HaCaT keratinocytes on exposure to Propionibacterium acnes. |
| Grantee: | Daisy Machado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/07808-7 - Use of graphen and carbon nanotubes as adjuvants in vaccine based in outer membrane vesicles from Neisseria meningitidis |
| Grantee: | Gabriel Piccirillo Gomide |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/15046-3 - Study of the genome and metabolome of the Haemophilus influenzae strains causing the Brazilian purpuric fever |
| Grantee: | Marcelo Lancellotti |
| Support Opportunities: | Regular Research Grants |