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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unraveling the molecular mechanisms underlying interactions between caseins and lutein

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Author(s):
Mantovani, Raphaela Araujo [1] ; Hamon, Pascaline [2] ; Rousseau, Florence [2] ; Tavares, Guilherme M. [1] ; Mercadante, Adriana Zerlotti [1] ; Croguennec, Thomas [2] ; Bouhallab, Said [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil
[2] STLO, Inst Agro, INRAE, F-35042 Rennes - France
Total Affiliations: 2
Document type: Journal article
Source: Food Research International; v. 138, n. B DEC 2020.
Web of Science Citations: 0
Abstract

Understanding the food protein binding to bioactive compounds is of utmost importance for the development of efficient protein-based delivery systems. The binding of lutein to sodium caseinate (NaCas) or native casein micelle (PPCN) was investigated at pH 7 to evaluate the effect of casein supramolecular structures on the interaction. Fluorescence quenching, UV-vis spectroscopy, and dynamic light scattering were carried out. Under the medium conditions of interaction analysis (DMSO-water and ethanol-water), lutein exists as H-type aggregates. The investigation of lutein/casein interaction showed a predominantly static mechanism of fluorescence quenching and the presence of two fluorophore populations on NaCas and PPCN, but only one accessible to lutein. Moreover, the Scatchard plot indicated that lutein interacted with both caseins in one binding site. The interaction of lutein with caseins occurred with binding constant K-b of 10(5) M-1, regardless of casein supramolecular structure. (AU)

FAPESP's process: 17/26237-8 - Understanding how protein-carotenoid interactions influence emulsion stability and carotenoid bioacessibility
Grantee:Raphaela de Araujo Mantovani
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/18374-8 - Understanding interactions between caseins and carotenoids at a molecular level
Grantee:Raphaela de Araujo Mantovani
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 17/09214-4 - How do structural changes of dairy proteins affect the functionality of bioactive compounds in complex food matrices?
Grantee:Guilherme Miranda Tavares
Support type: Research Grants - Young Investigators Grants