Novel Colloidal Nanocarrier of Cetylpyridinium Chloride: Antifungal Activities on Candida Species and Cytotoxic Potential on Murine Fibroblasts

Author(s)

Araujo, Heitor Ceolin ^[1] ; Arias, Lais Salomao ^[1] ; Morais Caldeirao, Anne Caroline ^[2] ; de Freitas Assumpcao, Lanay Caroline ^[3] ; Morceli, Marcela Grigoletto ^[3] ; de Souza Neto, Francisco Nunes ^[1] ; de Camargo, Emerson Rodrigues ^[4] ; Penha Oliveira, Sandra Helena ^[5] ; Pessan, Juliano Pelim ^[1] ; Monteiro, Douglas Roberto ^{[2, 3]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Western Sao Paulo UNOESTE, Grad Program Dent GPD Masters Degree, BR-19050920 Presidente Prudente, SP - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Sch Dent, BR-19050920 Presidente Prudente, SP - Brazil

^[4] Fed Univ Sao Carlos UFSCar, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[5] Sao Paulo State Univ Unesp, Sch Dent, Dept Basic Sci, BR-16015050 Aracatuba, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020.
• Web of Science Citations: 0

Abstract

Nanocarriers have been used as alternative tools to overcome the resistance of Candida species to conventional treatments. This study prepared a nanocarrier of cetylpyridinium chloride (CPC) using iron oxide nanoparticles (IONPs) conjugated with chitosan (CS), and assessed its antifungal and cytotoxic effects. CPC was immobilized on CS-coated IONPs, and the nanocarrier was physico-chemically characterized. Antifungal effects were determined on planktonic cells of Candida albicans and Candida glabrata (by minimum inhibitory concentration (MIC) assays) and on single- and dual-species biofilms of these strains (by quantification of cultivable cells, total biomass and metabolic activity). Murine fibroblasts were exposed to different concentrations of the nanocarrier, and the cytotoxic effect was evaluated by MTT reduction assay. Characterization methods confirmed the presence of a nanocarrier smaller than 313 nm. IONPs-CS-CPC and free CPC showed the same MIC values (0.78 mu g mL(-1)). CPC-containing nanocarrier at 78 mu g mL(-1) significantly reduced the number of cultivable cells for all biofilms, surpassing the effect promoted by free CPC. For total biomass, metabolic activity, and cytotoxic effects, the nanocarrier and free CPC produced statistically similar outcomes. In conclusion, the IONPs-CS-CPC nanocarrier was more effective than CPC in reducing the cultivable cells of Candida biofilms without increasing the cytotoxic effects of CPC, and may be a useful tool for the treatment of oral fungal infections. (AU)

• FAPESP's process: 17/24416-2 - Synthesis and antibiofilm effect of two new carrier nanosystems of antifungal drugs
• Grantee: Douglas Roberto Monteiro
• Support Opportunities: Regular Research Grants