| Full text | |
| Author(s): Show less - |
Batista, Luis F. S.
[1]
;
Torrecilha, Rafaela B. P.
[2]
;
Silva, Rafaela B.
[3]
;
Utsunomiya, Yuri T.
[4]
;
Silva, Thais B. F.
[1]
;
Tomokane, Thaise Y.
[1]
;
Pacheco, Acacio D.
[5]
;
Bosco, Anelise M.
[5]
;
Paulan, Silvana C.
[4]
;
Rossi, Claudio N.
[6]
;
Costa, Gustavo N. O.
[2]
;
Marcondes, Mary
[5]
;
Ciarlini, Paulo C.
[5]
;
Nunes, Caris M.
[4]
;
Matta, Vania L. R.
[1]
;
Laurenti, Marcia D.
[1]
Total Authors: 16
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Lab Patol Doencas Infecciosas, BR-01246903 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Salvador, Escola Saude, BR-41720200 Salvador, BA - Brazil
[4] Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16015050 Aracatuba, SP - Brazil
[5] Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, BR-16015050 Aracatuba, SP - Brazil
[6] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Veterinary Parasitology; v. 288, DEC 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Visceral leishmaniasis (VL) is marked by hyperactivation of a humoral response secreting high quantity of immunoglobulins (Igs) that are inaccessible to intracellular parasites. Here we investigated the contributions of the antibody response to the canine leishmaniasis pathogenesis. Using correlation and genome-wide association analysis, we investigated the relationship of anti-Leishmania infantum immunoglobulin classes levels with parasite burden, clinical response, renal/hepatic biochemical, and oxidative stress markers in dogs from endemic areas of VL. Immunoglobulin G (IgG) and IgA were positively correlated with parasite burden on lymph node and blood. Increased IgG, IgA and IgE levels were associated with severe canine leishmaniasis (CanL) whereas IgM was elevated in uninfected exposed dogs. Correlations of IgM, IgG and IgA with creatinine, urea, AST and ALT levels in the serum were suggested an involvement of those Igs with renal and hepatic changes. The correlogram of oxidative radicals and antioxidants revealed a likely relationship of IgM, IgG and IgA with oxidative stress and lipid pemxidation in the blood, suggested as mechanisms mediating tissue damage and CanL worsening. The gene mapping on chromosomal segments associated with the quantitative variation of immunoglobulin classes identified genetic signatures involved with reactive oxygen species generation, phagolysosome maturation and rupture, free iron availability, Thl/Th2 differenciation and, immunoglobulin clearance. The findings demonstrated the roles of the antibody response as resistance or susceptibility markers and mediators of CanL pathogenesis. In addition we pinpointed candidate genes as potential targets for the therapy against the damage caused by exacerbated antibody response and parasitism in VL. (AU) | |
| FAPESP's process: | 12/05847-9 - Clinical, immunological evaluation and their correlation with genetic markers in canine visceral leishmaniasis |
| Grantee: | Luís Fábio da Silva Batista |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/01095-8 - Use of next generation sequencing for the fine mapping of loci explaining variance in scrotal circumference in Nellore cattle |
| Grantee: | Yuri Tani Utsunomiya |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/50285-9 - Clinical, immunological features and its correlation with genetic markers in canine visceral leishmaniasis |
| Grantee: | Marcia Dalastra Laurenti |
| Support Opportunities: | Regular Research Grants |