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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synthetic chlorin derivative self-prevented from aggregation: Behavior in homogeneous medium for PDT applications

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Estevao, Bianca Martins [1, 2] ; de Freitas, Camila Fabiano [1] ; Franciscato, Douglas Santana [1, 3] ; de Assis, Francisco Favaro [4, 5] ; de Oliveira, Kleber Thiago [5] ; Hioka, Noboru [1] ; Caetano, Wilker [1] ; Muniz, Edvani Curti [1, 6, 7]
Total Authors: 8
[1] Univ Estadual Maringa, Dept Chem, Av Colombo 5790, BR-87020900 Maringa, Parana - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Ciencia Mat, Grp Nanomed & Nanotoxicol, Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Inst Quim, Quim Supramol & Nanotecnol, Sao Paulo - Brazil
[4] Univ Fed Santa Catarina, Dept Chem, BR-88040900 Florianopolis, SC - Brazil
[5] Univ Fed Sao Carlos, Dept Quim, Lab Quim Bioorgan, Sao Carlos, SP - Brazil
[6] Univ Fed Piaui, Dept Chem, Campus Petronio Portella, BR-64049550 Teresina, Piaui - Brazil
[7] Univ Tecnol Fed Parana, Estr Pioneiros 3131, BR-86036370 Londrina, Parana - Brazil
Total Affiliations: 7
Document type: Journal article
Source: JOURNAL OF MOLECULAR LIQUIDS; v. 320, n. B DEC 15 2020.
Web of Science Citations: 0

Herein the great performance of a synthetic chlorin (Chlo) as photosensitizer (PS) in a homogeneous ethanol/water environment and its interaction with protein model are described. Excellent interaction with the bovine serum albumin (BSA) protein model was achieved and the experimental results revealed that Chlo, designed to have low aggregation level, showed great stability in mixtures up to 65% of water, whereas the formation of small self-aggregates may take place in mixtures with greater proportion of water. In addition, computational calculations have shown that temperature influences the conformational stability of the molecule with consequences in aggregation. Fluorescence and electronic absorption spectroscopies were used to study the interaction between Chlo and BSA under simulative physiological condition at different temperatures.The study of the interaction with a protein model is important in view of the development of new drugs and their pharmacokinetic behavior. The results indicated strong interaction between Chlo and BSA, which is a spontaneous process guided by hydrophobic forces (Delta G <0; Delta H >0; Delta S > 0).These results corroborate with the vision of a good PS lobe applied in Photodynamic Therapy (PDT) and Antimicrobial Photodynamic Therapy (aPDT). (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/06532-4 - Cross-coupling reactions on the synthesis of new photosensitizers with high conjugation
Grantee:Kleber Thiago de Oliveira
Support type: Regular Research Grants
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 10/12545-3 - Total synthesis of hydrosoluble benzopophyrin derivatives with potential application in photodynamic therapy
Grantee:Francisco Fávaro de Assis
Support type: Scholarships in Brazil - Master