Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gut-licensed IFN gamma(+) NK cells drive LAMP1(+)TRAIL(+) anti-inflammatory astrocytes

Full text
Author(s):
Show less -
Sanmarco, Liliana M. [1] ; Wheeler, Michael A. [2, 1] ; Gutierrez-Vazquez, Cristina [1] ; Polonio, Carolina Manganeli [1] ; Linnerbauer, Mathias [1] ; Pinho-Ribeiro, Felipe A. [3] ; Li, Zhaorong [2, 1] ; Giovannoni, Federico [1] ; Batterman, Katelyn V. [4] ; Scalisi, Giulia [1] ; Zandee, Stephanie E. J. [5, 6] ; Heck, Evelyn S. [1] ; Alsuwailm, Moneera [1, 7] ; Rosene, Douglas L. [4] ; Becher, Burkhard [8] ; Chiu, Isaac M. [3] ; Prat, Alexandre [5, 6] ; Quintana, Francisco J. [2, 1]
Total Authors: 18
Affiliation:
[1] Harvard Med Sch, Brigham & Womens Hosp, Ann Romney Ctr Neurol Dis, Boston, MA 02115 - USA
[2] Broad Inst MIT & Harvard, Cambridge, MA 02142 - USA
[3] Harvard Med Sch, Dept Immunol, Boston, MA 02115 - USA
[4] Boston Univ, Sch Med, Dept Anat & Neurobiol, Boston, MA 02118 - USA
[5] CHUM, Ctr Rech, Neuroimmunol Unit, Montreal, PQ - Canada
[6] Univ Montreal, Fac Med, Dept Neurosci, Montreal, PQ - Canada
[7] King Abdulaziz City Sci & Technol KACST, Joint Ctr Excellence Program, Ctr Excellence Biomed, Riyadh - Saudi Arabia
[8] Univ Zurich, Inst Expt Immunol, Zurich - Switzerland
Total Affiliations: 8
Document type: Journal article
Source: Nature; v. 590, n. 7846 JAN 2021.
Web of Science Citations: 1
Abstract

Astrocytes are glial cells that are abundant in the central nervous system (CNS) and that have important homeostatic and disease-promoting functions(1). However, little is known about the homeostatic anti-inflammatory activities of astrocytes and their regulation. Here, using high-throughput flow cytometry screening, single-cell RNA sequencing and CRISPR-Cas9-based cell-specific in vivo genetic perturbations in mice, we identify a subset of astrocytes that expresses the lysosomal protein LAMP1(2) and the death receptor ligand TRAIL(3). LAMP1(+)TRAIL(+) astrocytes limit inflammation in the CNS by inducing T cell apoptosis through TRAIL-DR5 signalling. In homeostatic conditions, the expression of TRAIL in astrocytes is driven by interferon-gamma (IFN gamma) produced by meningeal natural killer (NK) cells, in which IFN gamma expression is modulated by the gut microbiome. TRAIL expression in astrocytes is repressed by molecules produced by T cells and microglia in the context of inflammation. Altogether, we show that LAMP1(+)TRAIL(+) astrocytes limit CNS inflammation by inducing T cell apoptosis, and that this astrocyte subset is maintained by meningeal IFN gamma(+) NK cells that are licensed by the microbiome. (AU)

FAPESP's process: 19/13731-0 - MicroRNAs, neurodevelopment and antiviral immune response: how they can be connected?
Grantee:Carolina Manganeli Polonio
Support type: Scholarships abroad - Research Internship - Doctorate