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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System

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Author(s):
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de Toledo, Luciani Gaspar [1] ; dos Santos Ramos, Matheus Aparecido [1] ; da Silva, Patricia Bento [2] ; Rodero, Camila Fernanda [3] ; Gomes, Veridiana de Sa [1] ; da Silva, Anderson Noronha [1] ; Pavan, Fernando Rogerio [1] ; da Silva, Isabel Cristiane [1] ; Oda, Fernando Bombarda [4] ; Flumignan, Danilo Luis [5] ; dos Santos, Andre Gonzaga [4] ; Chorilli, Marius [3] ; Gottardo de Almeida, Margarete Teresa [6] ; Bauab, Tais Maria [1]
Total Authors: 14
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Campus Araraquara, BR-14800903 Sao Paulo - Brazil
[2] Univ Brasilia UnB, Inst Biol Sci, Dept Genet & Morphol, Brasilia, DF - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drug & Med, Sao Paulo - Brazil
[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Nat Act Principles & Toxicol, Sao Paulo - Brazil
[5] Sao Paulo Fed Inst Educ Sci & Technol IFSP, Sao Paulo - Brazil
[6] Fac Med Sao Jose do Rio Preto FAMERP, Dept Infect Dis, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 15, p. 10481-10497, 2020.
Web of Science Citations: 0
Abstract

Purpose: Vulvovaginal candidiasis (VVC) is an opportunistic fungal infection that adversely affects a woman's health, due to unpleasant symptoms, therapeutic challenges, and the emergence of resistant strains. The association of natural products and nanotechnology is important to improve the antifungal potential of medicinal plants. We aimed to evaluate the in vitro and in vivo anti-Candida albicans activity of unloaded (EO) and loaded (ME+EO) essential oil of Cymbopogon nardus in the microemulsion (ME). Methods: The chemical analysis of the EO was performed by gas chromatography-mass spectrometry. The ME and ME+EO were characterized by scattering, zeta potential, polarized light microscopy, rheological assays, mucoadhesiveness and transmission electronic microscopy. The in vitro antifungal activity of the EO and ME+EO were evaluated by microdilution technique. The toxicity of EO and ME+EO was analyzed on human cell line HaCat and using alternative model assay with Artemia salina. The experimental in vivo VVC was performed in female mice (C57BL/6). Results: The main compounds of the EO were found to be citronellal, geranial, geraniol, citronellol, and neral. The formulations exhibited suitable size, homogeneity, negative charge, isotropic behavior, highly organized structure, and pseudoplastic behavior, for vaginal application. TEM photomicrographs showed possible EO droplets inside the spherical structures. The EO, when loaded into the ME, exhibited an improvement in its antifungal action against C. albicans. The EO was not toxic against brine shrimp nauplii. An in vivo VVC assay showed that the use of the ME significantly improved the action of the EO, since only the ME+EO promoted the eradication of the fungal vaginal infection on the third day of treatment. Conclusion: The EO and ME+EO are promising alternatives for the control of fungal infections caused by C. albicans, once the use of nanotechnology significantly improved the antifungal action of the EO, especially in an in vivo model of VVC. (AU)

FAPESP's process: 16/08559-5 - Chemical analyze and therapeutic prospection of Syngonanthus nitens (Bong.) Ruhland and Cymbopogon nardus (L.) Rendle loaded into nanotechnological systems applied to vulvovaginal candidiasis treatment
Grantee:Taís Maria Bauab
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
Grantee:Vanderlan da Silva Bolzani
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 15/23959-7 - Cymbopogon nardus (L.) Rendle (citronella): chemical-biological prospecting of essential oil highlighted the biofilm study and control of vulvovaginal candidiasis
Grantee:Luciani Gaspar de Toledo
Support Opportunities: Scholarships in Brazil - Doctorate