| Full text | |
| Author(s): Show less - |
Spangenberg, Victor
[1]
;
Arakelyan, Marine
[2]
;
Galoyan, Eduard
[3]
;
Martirosyan, Irena
[4]
;
Bogomazova, Alexandra
[5]
;
Martynova, Elena
[6]
;
de Bello Cioffi, Marcelo
[7]
;
Liehr, Thomas
[8]
;
Al-Rikabi, Ahmed
[8]
;
Osipov, Fedor
[3]
;
Petrosyan, Varos
[3]
;
Kolomiets, Oxana
[1]
Total Authors: 12
|
| Affiliation: | [1] Vavilov Inst Gen Genet RAS, Moscow 119991 - Russia
[2] Yerevan State Univ, Dept Zool, Yerevan - Armenia
[3] Severtsov Inst Ecol & Evolut RAS, Moscow - Russia
[4] RAS, Inst Gene Biol, Moscow - Russia
[5] Fed Med Biol Agcy, Fed Res & Clin Ctr Phys Chem Med, Moscow - Russia
[6] Skolkovo Inst Sci & Technol, Moscow - Russia
[7] Univ Fed Sao Carlos, Lab Citogenet Peixes, Sao Carlos, SP - Brazil
[8] Friedrich Schiller Univ, Inst Human Genet, Jena Univ Hosp, Jena - Germany
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Molecular Reproduction and Development; v. 88, n. 2 JAN 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Parthenogenetic species of Caucasian rock lizards of the genus Darevksia are important evidence for reticulate evolution and speciation by hybridization in vertebrates. Female-only lineages formed through interspecific hybridization have been discovered in many groups. Nevertheless, critical mechanisms of oogenesis and specifics of meiosis that provide long-term stability of parthenogenetic species are still unknown. Here we report cytogenetic characteristics of somatic karyotypes and meiotic prophase I nuclei in the diploid parthenogenetic species Darevskia unisexualis from the new population ``Keti{''} in Armenia which contains an odd number of chromosomes 2n = 37, instead of the usual 2n = 38. We revealed 36 acrocentric chromosomes and a single metacentric autosomal chromosome, resulting from Robertsonian translocation. Comparative genomic hybridization revealed that chromosome fusion occurred between two chromosomes inherited from the maternal species, similar to another parthenogenetic species D. rostombekowi. To trace the chromosome behaviour in meiosis, we performed an immunocytochemical study of primary oocytes' spread nuclei and studied chromosome synapsis during meiotic prophase I in D. unisexualis based on analysis of synaptonemal complexes (SCs). We found meiotic SC-trivalent composed of one metacentric and two acrocentric chromosomes. We confirmed that the SC was assembled between homeologous chromosomes inherited from two parental species. Immunostaining of the pachytene and diplotene nuclei revealed a mismatch repair protein MLH1 loaded to all autosomal SC bivalents. Possible mechanisms of meiotic recombination between homeologous chromosomes are discussed. (AU) | |
| FAPESP's process: | 18/22033-1 - An intercontinental approach for the investigation of chromosome evolution, genetic diversity and biogeography in the order Osteoglossiformes (Teleostei: Osteoglossomorpha). Part III |
| Grantee: | Marcelo de Bello Cioffi |
| Support Opportunities: | Regular Research Grants |