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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pregnancy decreases O-GlcNAc-modified proteins in systemic arteries of normotensive and spontaneously hypertensive rats

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Troiano, Jessica A. [1, 2] ; Potje, Simone R. [3] ; Graton, Murilo E. [1, 2] ; Silva, Daniela S. [2] ; da Costa, Rafael M. [4] ; Tostes, Rita C. [5] ; Antoniali, Cristina [1, 2]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Programa Posgrad Multictr Ciencias Fisiol, SBFis, Aracatuba, SP - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ribeirao Preto, SP - Brazil
[4] Univ Fed Goias, Special Acad Unit Hlth Sci, Jatai, Go - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Life Sciences; v. 266, FEB 1 2021.
Web of Science Citations: 0

Aim: We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats. Main methods: O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR). Vascular O-GlcNAc-modified proteins, OGlcNAcase (OGA) and O-GlcNAc transferase (OGT) expression, and OGA activity were analyzed. Concentration-response to phenylephrine (PE) curves were constructed for arteries with and without endothelium. Arteries were treated with vehicle or PugNAc (OGA inhibitor, 100 mu mol/L) in the presence of L-NAME (NOS inhibitor, 100 mu mol/L). Key findings: The content of vascular O-GlcNAc-modified proteins was lower, OGT and OGA expression did not change, and OGA activity was higher in arteries of P-Wistar rats and P-SHR compared to arteries of NP-groups. Reactivity to PE increased in arteries of P-Wistar rats treated with PugNAc compared to vehicle. O-GlcNAcylation of eNOS decreased in P-SHR compared to NP-SHR. PugNAc partially inhibited the effects of endothelium removal and L-NAME on reactivity to PE in arteries of P-Wistar rats. However, PugNAc did not alter reactivity to PE in arteries of P-SHR. Our data showed that pregnancy decreased the content of vascular O-GlcNAc-modified proteins. Significance: Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats. In P-SHR, altered OGA activity may lower the content of O-GlcNAc-modified proteins, but decreased OGT activity seems a potential mechanism to reduce glycosylation. (AU)

FAPESP's process: 16/22180-9 - Study of post-translational modification mechanisms that increase the bioavailability of nitric oxide in blood vessels of normotensive and spontaneously hypertensive rats SHR at the end of pregnancy.
Grantee:Cristina Antoniali Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 15/09373-0 - Evaluation of post-translational modification mechanisms of endothelial Nitric Oxide Synthase (eNOS) associated with the nitric oxide bioavailability in Spontaneously Hypertensive Rats (SHR) in the end of pregnancy
Grantee:Jéssica Antonini Troiano
Support Opportunities: Scholarships in Brazil - Doctorate