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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Correlation between SARS-COV-2 antibody screening by immunoassay and neutralizing antibody testing

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Mendrone-Junior, Alfredo [1, 2] ; Dinardo, Carla Luana [1, 3] ; Ferreira, Suzete Cleuza [1] ; Nishya, Anna [1] ; Salles, Nanci Alves [1] ; de Almeida Neto, Cesar [1] ; Hamasaki, Debora Toshei [1] ; Facincani, Tila [1] ; de Oliveira Alves, Lucas Bassolli [4] ; Machado, Rafael Rahal Guaragna [5] ; Araujo, Danielle Bastos [5] ; Durigon, Edison Luiz [5, 6] ; Rocha, Vanderson [1, 2, 4] ; Sabino, Ester Cerdeira [3]
Total Authors: 14
[1] Undacao Pro Sangue Hemoctr Sao Paulo, Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Lab Med Invest Pathogenesis & Directed Therapy On, HCFMUSP, Sao Paulo - Brazil
[3] FMUSP, Inst Med Trop, Sao Paulo - Brazil
[4] Serv Hematol Hemoterapia & Terapia Celular HCFMUS, Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
[6] Sci Platform Pasteur USP, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Transfusion; v. 61, n. 4 JAN 2021.
Web of Science Citations: 0

Background The efficacy of convalescent plasma (CP), an alternative for the treatment of COVID-19, depends on high titers of neutralizing antibodies (nAbs), but assays for quantifying nAbs are not widely available. Our goal was to develop a strategy to predict high titers of nAbs based on the results of anti-SARS-CoV-2 immunoassays and the clinical characteristics of CP donors. Study Design and Methods A total of 214 CP donors were enrolled and tested for the presence of anti-SARS-CoV-2 antibodies (IgG) using two commercial immunoassays: EUROIMMUN (ELISA) and Abbott (Chemiluminescence). Quantification of nAbs was performed using the Cytopathic Effect-based Virus Neutralization test. Three criteria for identifying donors with nAbs >= 1:160 were tested: - C1: Curve ROC; - C2: Conditional decision tree considering only the IA results and - C3: Conditional decision tree including both the IA results and the clinical variables. Results The performance of the immunoassays was similar referring to both S/CO and predictive value for identifying nAbs titers >= 1:160. Regarding the studied criteria for identifying CP donors with high nAbs titers: (a) C1 showed 76.1% accuracy if S/CO = 4.65, (b) C2 presented 76.1% accuracy if S/CO >= 4.57 and (c) C3 had 71.6% accuracy if S/CO was >= 4.57 or if S/CO was between 2.68-4.57 and the last COVID-19-related symptoms were recent (within 19 days). Conclusion SARS-CoV-2 IgG immunoassays (S/CO) can be used to predict high anti-SARS-CoV-2 nAbs titers. This study has proposed different criteria for identifying donors with >= 1:160 nAbs titers, all with high efficacy. (AU)

FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
Grantee:Edison Luiz Durigon
Support type: Regular Research Grants
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/24769-2 - Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization
Grantee:Rafael Rahal Guaragna Machado
Support type: Scholarships in Brazil - Doctorate (Direct)