Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential regulation of LRRC37A2 in gastric cancer by DNA methylation

Full text
Author(s):
Show less -
Wisnieski, Fernanda [1, 2] ; Geraldis, Jaqueline Cruz [1] ; Santos, Leonardo Caires [1] ; Leal, Mariana Ferreira [1, 3] ; Calcagno, Danielle Queiroz [3] ; Gigek, Carolina Oliveira [4] ; Chen, Elizabeth Suchi [1] ; Anauate, Ana Carolina [1] ; Artigiani, Ricardo [4] ; Demachki, Samia [3] ; Assumpcao, Paulo Pimentel [3] ; Lourenco, Laercio Gomes [5] ; Arasaki, Carlos Haruo [5] ; Krainer, Julie [6] ; Pabinger, Stephan [6] ; Burbano, Rommel Rodriguez [3, 7] ; Cardoso Smith, Marilia Arruda [1]
Total Authors: 17
Affiliation:
[1] Univ Fed Sao Paulo, Dept Morfol & Genet, Disciplina Genet, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Med, Disciplina Gastroenterol, Rua Loefgreen 1726, BR-04040002 Sao Paulo - Brazil
[3] Univ Fed Para, Belem, Para - Brazil
[4] Univ Fed Sao Paulo, Dept Patol, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Cirurgia, Disciplina Gastroenterol Cirurg, Sao Paulo - Brazil
[6] Austrian Inst Technol, Ctr Hlth & Bioresources, Vienna - Austria
[7] Hosp Ophir Loyola, Lab Biol Mol, Belem, Para - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Epigenetics; FEB 2021.
Web of Science Citations: 0
Abstract

Gastric cancer (GC) is one of the leading types of fatal cancer worldwide. Epigenetic manipulation of cancer cells is a useful tool to better understand gene expression regulatory mechanisms and contributes to the discovery of novel biomarkers. Our research group recently reported a list of 83 genes that are potentially modulated by DNA methylation in GC cell lines. Herein, we further explored the regulation of one of these genes, LRRC37A2, in clinical samples. LRRC37A2 expression was evaluated by RT-qPCR, and DNA methylation was studied using next-generation bisulphite sequencing in 36 GC and paired adjacent nonneoplastic tissue samples. We showed that both reduced LRRC37A2 mRNA levels and increased LRRC37A2 exon methylation were associated with undifferentiated and poorly differentiated tumours. Moreover, LRRC37A2 gene expression and methylation levels were inversely correlated at the +45 exon CpG site. We suggest that DNA hypermethylation may contribute to reducing LRRC37A2 expression in undifferentiated and poorly differentiated GC. Therefore, our results show how some genes may be useful to stratify patients who are more likely to benefit from epigenetic therapy. (AU)

FAPESP's process: 09/07145-9 - Genetic and epigenetic aspects in gastric carcinogenesis
Grantee:Marilia de Arruda Cardoso Smith
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/06227-8 - DNA methylation analysis in transcriptional regulation comprehension of relevant genes in gastric carcinogesis
Grantee:Jaqueline Cruz Geraldis
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/11174-1 - Genetic and epigenetic evaluation in gastric cancer
Grantee:Danielle Queiroz Calcagno
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/19953-6 - Epigenetically modulated genes may constitute therapeutic targets in patients with gastric cancer?
Grantee:Fernanda Wisnieski
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 07/02470-3 - Differential phospho and glyco proteomic analysis in gastric adenocarcinoma and the eventual correlation with clinic-pathological parameters of prognosis
Grantee:Mariana Ferreira Leal
Support type: Scholarships in Brazil - Doctorate