A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for percutaneous implants devices

Author(s)

Verza, Beatriz S. ^[1] ; van den Beucken, Jeroen J. J. P. ^[2] ; Brandt, V, Joao ; Jafelicci Junior, Miguel ^[3] ; Barao, Valentim A. R. ^[4] ; Piazza, Rodolfo D. ^[3] ; Tagit, Oya ^{[5, 6]} ; Spolidorio, Denise M. P. ^[7] ; Vergani, Carlos Eduardo ^[1] ; de Avila, Erica D. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Dent Mat & Prosthodont, Humaita 1680, Araraquara, SP - Brazil

^[2] Radboudumc, Regenerat Biomat, Philips Van Leydenlaan 25, Nijmegen - Netherlands

^[3] Brandt, Joao, V, Sao Paulo State Univ UNESP, Inst Chem, Dept Phys Chem, BR-14801970 Araraquara, SP - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[5] Radboudumc, Dept Tumor Immunol, Geert Grootepl Zuid 28, Nijmegen - Netherlands

^[6] Radboud Inst Mol Life Sci RIMLS, Geert Grootepl Zuid 28, Nijmegen - Netherlands

^[7] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 257, APR 1 2021.
• Web of Science Citations: 0

Abstract

This study demonstrated a drug-delivery system with anionic beta cyclodextrin (beta-CD) complexes to retain tetracycline (TC) and control its release from multilayers of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL) in a ten double layers ({[}PAA/PLL](10)) coating onto titanium. The drug-delivery capacity of the multilayer system was proven by controlled drug release over 15 days and sustained released over 30 days. Qualitative images confirmed TC retention within the layer-by-layer (LbL) over 30 days of incubation. Antibacterial activity of TC/anionic beta-CD released from the LbL was established against Staphylococcus aureus species. Remarkably, {[}PAA/ PLL] 10/TC/anionic beta-CD antibacterial effect was sustained even af ter 30 days of incubation. The non-cytotoxic effect of the multilayer system revealed normal human gingival fibroblast growth. It is expected that this novel approach and the chemical concept to improve drug incorporation into the multilayer system will open up possibilities to make the drug release system more applicable to implantable percutaneous devices. (AU)

• FAPESP's process: 18/19345-1 - Improving method of drug-delivery system with ionic-cyclodextrin and their inclusion complexes with antimicrobial agent into the polymeric multilayers
• Grantee: Beatriz Severino Verza
• Support Opportunities: Scholarships in Brazil - Scientific Initiation