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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CXCL5, CXCL8, and CXCL10 regulation by bacteria and mechanical forces in periodontium

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Author(s):
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Rath-Deschner, Birgit [1] ; Memmert, Svenja [1, 2] ; Damanaki, Anna [3] ; de Molon, Rafael S. [4] ; Nokhbehsaim, Marjan [2] ; Eick, Sigrun [5] ; Kirschneck, Christian [6] ; Cirelli, Joni A. [4] ; Deschner, James [3] ; Jaeger, Andreas [1] ; Nogueira, Andressa V. B. [3]
Total Authors: 11
Affiliation:
[1] Univ Bonn, Ctr Dentomaxillofacial Med, Dept Orthodont, Welschnonnenstr 17, D-53111 Bonn - Germany
[2] Univ Bonn, Ctr Dentomaxillofacial Med, Sect Expt Dentomaxillofacial Med, Welschnonnenstr 17, D-53111 Bonn - Germany
[3] Johannes Gutenberg Univ Mainz, Dept Periodontol & Operat Dent, Univ Med Ctr, D-55131 Mainz - Germany
[4] Sao Paulo State Univ, UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP - Brazil
[5] Univ Bern, Dept Periodontol, Lab Oral Microbiol, CH-3012 Bern - Switzerland
[6] Univ Med Ctr Regensburg, Dept Orthodont, D-93053 Regensburg - Germany
Total Affiliations: 6
Document type: Journal article
Source: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER; v. 234, MAR 2021.
Web of Science Citations: 1
Abstract

Objective: The aim of the present study was to evaluate the expressions of CXCL5, CXCL8, and CXCL10 in periodontal cells and tissues in response to microbial signals and/or biomechanical forces. Methods: Human gingival biopsies from inflamed and healthy sites were used to examine the chemokine expressions and protein levels by real-time PCR and immunohistochemistry. The chemokines were also investigated in gingival biopsies from rats submitted to experimental periodontitis and/or tooth movement. Furthermore, chemokine levels were determined in human periodontal fibroblasts stimulated by the periodontopathogen Fusobacterium nucleatum and/or constant tensile forces (CTS) by real-time PCR and ELISA. Additionally, gene expressions were evaluated in periodontal fibroblasts exposed to F. nucleatum and/or CTS in the presence and absence of a MAPK inhibitor by real-time PCR. Results: Increased CXCL5, CXCL8, and CXCL10 levels were observed in human and rat gingiva from sites of inflammation as compared with periodontal health. The rat experimental periodontitis caused a significant (p < 0.05) increase in alveolar bone resorption, which was further enhanced when combined with tooth movement. In vitro, F. nucleatum caused a significant upregulation of CXCL5, CXCL8, and CXCL10 at 1 day. Once the cells were exposed simultaneously to F. nucleatum and CTS, the chemokines regulation was significantly enhanced. The transcriptional findings were also observed at protein level. Pre-incubation with the MEK1/2 inhibitor significantly (p < 0.05) inhibited the stimulatory actions of F. nucleatum either alone or in combination with CTS on the expression levels of CXCL5, CXCL8, and CXCL10 at 1 d. Conclusions: Our data provide original evidence that biomechanical strain further increases the stimulatory actions of periodontal bacteria on the expressions of these chemokines. Therefore, biomechanical loading in combination with periodontal infection may lead to stronger recruitment of immunoinflammatory cells to the periodontium, which might result in an aggravation of periodontal inflammation and destruction. (C) 2020 Elsevier GmbH. All rights reserved. (AU)

FAPESP's process: 17/07137-2 - Effects of obesity on periodontal tissues subjected to biomechanical forces
Grantee:Joni Augusto Cirelli
Support Opportunities: Regular Research Grants