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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unravelling the involvement of cilevirus p32 protein in the viral transport

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Author(s):
Leastro, Mikhail Oliveira [1, 2] ; Freitas-Astua, Juliana [3, 1] ; Kitajima, Elliot Watanabe [4] ; Pallas, Vicente [2] ; Sanchez-Navarro, Jesus A. [2]
Total Authors: 5
Affiliation:
[1] Inst Biol, Unidade Lab Referencia Biol Mol Aplicada, Sao Paulo, SP - Brazil
[2] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia - Spain
[3] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA - Brazil
[4] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 FEB 3 2021.
Web of Science Citations: 2
Abstract

Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. (AU)

FAPESP's process: 15/10249-1 - Studies of infective processes of Citrus leprosis virus (CiLV-C), with emphasis in the construction of infectious clone and characterization of viral proteins.
Grantee:Mikhail Oliveira Leastro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies
Grantee:Juliana de Freitas Astúa
Support Opportunities: Regular Research Grants
FAPESP's process: 17/19898-8 - The involvement of the movement protein (MP) of Citrus leprosis-associated viruses with the natural restraint of systemic viral spread in the plant.
Grantee:Mikhail Oliveira Leastro
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor