Active Antioxidant Phenolics from Brazilian Red Pr... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Active Antioxidant Phenolics from Brazilian Red Propolis: An Optimization Study for Their Recovery and Identification by LC-ESI-QTOF-MS/MS

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Author(s):
Vieira de Morais, Daniel [1] ; Rosalen, Pedro Luiz [2] ; Ikegaki, Masaharu [2] ; de Souza Silva, Anna Paula [3] ; Massarioli, Adna Prado [3] ; de Alencar, Severino Matias [1, 3]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13416000 Piracicaba, SP - Brazil
[2] Univ Fed Alfenas, Fac Pharmaceut Sci, BR-37130001 Alfenas, MG - Brazil
[3] Univ Sao Paulo, Luiz De Queiroz Coll Agr, Dept Agri Food Ind Food & Nutr, BR-13416000 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ANTIOXIDANTS; v. 10, n. 2 FEB 2021.
Web of Science Citations: 0
Abstract

Brazilian red propolis (BRP) is a natural product widely known for its phenolic composition and strong antioxidant properties. In this study, we used the Box-Behnken Design (BBD) with Surface Response Methodology to optimize the extraction conditions for total phenolic content (TPC) and Trolox equivalent antioxidant capacity(TEAC) of bioactive phenolics from BRP. The extraction time, ethanol/water concentration and temperature, were tested. All variables had significant effects (p <= 0.05), with a desirability coefficient of 0.88. Under optimized conditions (90% ethanol at 80 degrees C for 30 min), the BRP extract showed a TPC of 129.00 +/- 2.16 mg GAE/g and a TEAC of 3471.76 +/- 53.86 mu mol TE/g. Moreover, FRAP and ORAC assays revealed that the optimized BRP extract had 1472.86 +/- 72.37 mu mol Fe2+/g and 4339.61 +/- 114.65 mu mol TE/gof dry weight, respectively. Thirty-two phenolic compounds were tentatively identified by LC-QTOF-ESI-MS/MS, of which thirteen were found for the first time in BRP, including four flavones, one flavanol, two flavanones, two chalcones, and four isoflavonoids. Thus, our results highlight the importance of BRP as a source of a wide variety of phenolic compounds with significant antioxidant properties. (AU)

FAPESP's process: 19/11248-0 - Production of Brazilian red propolis microcapsules for use as a natural preservative and development of functional foods: characterization, bioaccessibility and epithelial transport through Caco-2 cells
Grantee:Severino Matias de Alencar
Support Opportunities: Regular Research Grants