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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography

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Author(s):
Serafim, Jessica Aparecida [1] ; Silveira, Raiza Felismino [2] ; Vicente, Eduardo Festozo [1]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Sci & Engn, Tupa, SP - Brazil
[2] Sao Paulo State Univ Unesp, Sch Agr & Veterinarian Sci, Jaboticabal, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FOOD ANALYTICAL METHODS; v. 14, n. 7 FEB 2021.
Web of Science Citations: 0
Abstract

A rapid analytical method for simultaneous quantification of glucose (GLC) and seven short-chain fatty acids (SCFA) was developed for detection of ruminal fermentation parameters. The formic acid (FA), lactic acid (LA), acetic acid (AA), propionic acid (PA), butyric acid (BA), isovaleric acid (IVA), valeric acid (VA), and glucose were identified and quantified by high-performance liquid chromatography (HPLC) technique, using a reverse-phase column (C-18) and simultaneous detection in UV-Vis and Refraction Index (RID) detectors, linked in series. The method was validated following the requirements of selectivity, linearity, sensitivity, limit of detection, limit of quantification, accuracy, and precision. The developed method operates in isocratic and gradient modes, providing a better SCFA separation, which allows their determination with a high accuracy and repeatability. Therefore, this study provides a fast, reliable, accurate, straightforward, and efficient alternative method for analysis of ruminal fermentation parameters by liquid chromatography using both detectors simultaneously. (AU)

FAPESP's process: 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition
Grantee:Eduardo Festozo Vicente
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/10393-0 - Multi-user equipment approved in grant number 2016/00446-7: HPLC system
Grantee:Eduardo Festozo Vicente
Support Opportunities: Multi-user Equipment Program