Elderly Subjects Supplemented with L-Glutamine Shows an Improvement of Mucosal Immunity in the Upper Airways in Response to Influenza Virus Vaccination

Author(s):Show less -

Paixao, Vitoria ^[1] ; Almeida, Ewin B. ^[1] ; Amaral, Jonatas B. ^[1] ; Roseira, Tamaris ^{[2, 1]} ; Monteiro, Fernanda R. ^{[2, 1]} ; Foster, Roberta ^{[2, 1]} ; Sperandio, Adriane ^[2] ; Rossi, Marcelo ^[1] ; Amirato, Gislene R. ^[1] ; Santos, Carlos A. F. ^[3] ; Pires, Renier S. ^[4] ; Leal, Fabyano B. ^[5] ; Durigon, Edison L. ^{[5, 6]} ; Oliveira, Danielle B. L. ^{[7, 5]} ; Vieira, Rodolfo P. ^{[8, 9, 10]} ; Vaisberg, Mauro ^[1] ; Santos, Juliana M. B. ^[10] ; Bachi, Andre L. L. ^{[8, 1, 4]}

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Dept Otorhinolaryngol, ENT Lab, BR-04021001 Sao Paulo - Brazil

^[2] Method Fac Sao Paulo FAMESP, BR-04046200 Sao Paulo - Brazil

^[3] Paulista Sch Med EPM, Dept Med, Geriatry, BR-04023062 Sao Paulo - Brazil

^[4] Santo Amaro Univ UNISA, Postgrad Program Hlth Sci, BR-04743030 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, BR-05508060 Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sci Platform Pasteur, BR-05508060 Sao Paulo - Brazil

^[7] Hosp Israelita Albert Einstein, BR-05652900 Sao Paulo - Brazil

^[8] Brazilian Inst Teaching & Res Pulm & Exercise Imm, BR-12245520 Sao Paulo - Brazil

^[9] Univ Brasil, Postgrad Program Bioengn & Biomed Engn, BR-15600000 Sao Paulo - Brazil

^[10] Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human & Rehabil, BR-11015020 Santos, SP - Brazil

• Document type: Journal article
• Source: VACCINES; v. 9, n. 2 FEB 2021.
• Web of Science Citations: 1

Abstract

Background: Although glutamine is able to improve the immune response, its action in the upper airway immunity against the influenza virus vaccine remains unclear. Therefore, we aimed to evaluate the L-glutamine supplementation effect on the mucosal immune/inflammatory response of elderly subjects vaccinated against the influenza virus. Methods: Saliva sampling from 83 physically active elderly volunteers were collected pre- and 30 days after influenza virus vaccination and supplementation with L-glutamine (Gln, n = 42) or placebo (PL, n = 41). Results: Gln group showed higher salivary levels of interleukin (IL)-17, total secretory immunoglobulin A (SIgA), and specific-SIgA post-vaccination than values found pre-vaccination and in the PL group post-vaccination. Whereas higher salivary levels of IL-6 and IL-10 were observed post-vaccination in the Gln group, IL-37 levels were lower post-vaccination in both groups than the values pre-vaccination. Tumor necrosis factor (TNF)-alpha levels were unchanged. Positive correlations between IL-6 and IL-10 were found in all volunteer groups pre- and post-vaccination and also between IL-17 and IL-6 or IL-10 in the Gln group post-vaccination. A negative correlation between IL-37 and IL-10 was found pre- and post-vaccination in the PL group. Conclusion: Gln supplementation was able to modulate salivary cytokine profile and increase SIgA levels, both total and specific to the influenza virus vaccine, in physically active elderly subjects. (AU)

• FAPESP's process: 19/13188-4 - Study of the effects of glutamine supplementation on the immunophenotyping and activation profile of t and b lymphocytes from practicing or NON-EXERCISING seniors submitted to influenza virus vaccination
• Grantee: Tamaris Roseira dos Reis Pavao
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/13094-0 - Evaluation of the influence of combined physical exercise and L-glutamine supplementation on the inflammatory profile and response of specific antibodies to the vaccine against the influenza virus in elderly people
• Grantee: Fernanda Rodrigues Monteiro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/14115-0 - Influence of L-glutamine supplementation in the sarcopenia and immune/inflammatory response both systemic and in the respiratory tract of elderly practitioners or not of a regular program of combined physical exercises submitted to Influenza virus vaccine
• Grantee: André Luis Lacerda Bachi
• Support Opportunities: Regular Research Grants