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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Palmitic Acid Impairs Myogenesis and Alters Temporal Expression of miR-133a and miR-206 in C2C12 Myoblasts

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Author(s):
da Paixao, Ailma O. [1] ; Bolin, Anaysa Paola [1] ; Silvestre, Joao G. [2] ; Rodrigues, Alice Cristina [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Pharmacol, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 5 MAR 2021.
Web of Science Citations: 0
Abstract

Palmitic acid (PA), a saturated fatty acid enriched in high-fat diet, has been implicated in the development of sarcopenic obesity. Herein, we chose two non-cytotoxic concentrations to better understand how excess PA could impact myotube formation or diameter without inducing cell death. Forty-eight hours of 100 mu M PA induced a reduction of myotube diameter and increased the number of type I fibers, which was associated with increased miR-206 expression. Next, C2C12 myotube growth in the presence of PA was evaluated. Compared to control cells, 150 mu M PA reduces myoblast proliferation and the expression of MyoD and miR-206 and miR-133a expression, leading to a reduced number and diameter of myotubes. PA (100 mu M), despite not affecting proliferation, impairs myotube formation by reducing the expression of Myf5 and miR-206 and decreasing protein synthesis. Interestingly, 100 and 150 mu M PA-treated myotubes had a higher number of type II fibers than control cells. In conclusion, PA affects negatively myotube diameter, fusion, and metabolism, which may be related to myomiRs. By providing new insights into the mechanisms by which PA affects negatively skeletal muscle, our data may help in the discovery of new targets to treat sarcopenic obesity. (AU)

FAPESP's process: 15/24789-8 - MicroRNAs on adiponectin signalling: potential therapeutical targets of insulin resistance and insulin resistance linked diseases.
Grantee:Alice Cristina Rodrigues
Support Opportunities: Regular Research Grants
FAPESP's process: 18/07087-8 - The role of microRNAs in metabolic alterations promoted by diet-induced obesity or maternal obesity
Grantee:Alice Cristina Rodrigues
Support Opportunities: Regular Research Grants
FAPESP's process: 20/08049-2 - Circulating microRNAs as mediators of metabolic adaptation promoted by aerobic exercise in obesity
Grantee:Alice Cristina Rodrigues
Support Opportunities: Regular Research Grants