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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Long-term persistence of supernumerary B chromosomes in multiple species of Astyanax fish

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Zerbinato de Andrade Silva, Duilio Mazzoni [1] ; Ruiz-Ruano, Francisco J. [2, 3, 4] ; Utsunomia, Ricardo [5, 6] ; Martin-Pecina, Maria [2] ; Pena Castro, Jonathan [7, 8] ; Paccielli Freire, Paula [9, 1] ; Francisco Carvalho, Robson [1] ; Hashimoto, Diogo T. [10] ; Suh, Alexander [3, 4] ; Oliveira, Claudio [1] ; Porto-Foresti, Fabio [5] ; Ferreira Artoni, Roberto [7, 8] ; Foresti, Fausto [1] ; Camacho, Juan Pedro M. [2]
Total Authors: 14
Affiliation:
[1] Univ Estadual Paulista, Dept Biol Estrutural & Func, Inst Biociencias Botucatu, UNESP, BR-18618970 Botucatu, SP - Brazil
[2] Univ Granada, Dept Genet, Granada 18071 - Spain
[3] Uppsala Univ, Dept Organismal Biol Systemat Biol, Evolutionary Biol Ctr, SE-75236 Uppsala - Sweden
[4] Univ East Anglia, Sch Biol Sci, Norwich Res Pk, Norwich NR4 7TU, Norfolk - England
[5] Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, UNESP, Campus Bauru, BR-17033360 Bauru, SP - Brazil
[6] Univ Fed Rural Rio de Janeiro, Dept Genet, Inst Ciencias Biol & Saude, BR-23897000 Seropedica, RJ - Brazil
[7] Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, BR-84030900 Ponta Grossa, PR - Brazil
[8] Univ Fed Sao Carlos, Dept Genet & Evolucao, UFSCar, BR-13565905 Sao Carlos, SP - Brazil
[9] Univ Sao Paulo, Dept Imunol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, SP - Brazil
[10] Univ Estadual Paulista, Ctr Aquicultura, UNESP, Campus Jaboticabal, BR-14884900 Jaboticabal, SP - Brazil
Total Affiliations: 10
Document type: Journal article
Source: BMC Biology; v. 19, n. 1 MAR 19 2021.
Web of Science Citations: 0
Abstract

BackgroundEukaryote genomes frequently harbor supernumerary B chromosomes in addition to the ``standard{''} A chromosome set. B chromosomes are thought to arise as byproducts of genome rearrangements and have mostly been considered intraspecific oddities. However, their evolutionary transcendence beyond species level has remained untested.ResultsHere we reveal that the large metacentric B chromosomes reported in several fish species of the genus Astyanax arose in a common ancestor at least 4 million years ago. We generated transcriptomes of A. scabripinnis and A. paranae 0B and 1B individuals and used these assemblies as a reference for mapping all gDNA and RNA libraries to quantify coverage differences between B-lacking and B-carrying genomes. We show that the B chromosomes of A. scabripinnis and A. paranae share 19 protein-coding genes, of which 14 and 11 were also present in the B chromosomes of A. bockmanni and A. fasciatus, respectively. Our search for B-specific single-nucleotide polymorphisms (SNPs) identified the presence of B-derived transcripts in B-carrying ovaries, 80% of which belonged to nobox, a gene involved in oogenesis regulation. Importantly, the B chromosome nobox paralog is expressed >30x more than the A chromosome paralog. This indicates that the normal regulation of this gene is altered in B-carrying females, which could potentially facilitate B inheritance at higher rates than Mendelian law prediction.ConclusionsTaken together, our results demonstrate the long-term survival of B chromosomes despite their lack of regular pairing and segregation during meiosis and that they can endure episodes of population divergence leading to species formation. (AU)

FAPESP's process: 18/20610-1 - Study of the biogeography of the Brazilian coast using data population genetic data of marine catfish of the genera Cathorops and Genidens
Grantee:Claudio de Oliveira
Support type: Regular Research Grants
FAPESP's process: 14/09634-5 - Functional and evolutionary analysis of b chromosomes using the Astyanax paranae fish (Characiformes Characidae) as model
Grantee:Fausto Foresti
Support type: Regular Research Grants
FAPESP's process: 17/22447-8 - Comparative Genetic Activity on B Chromosomes of Astyanax species (Teleostei, Characiformes)
Grantee:Duílio Mazzoni Zerbinato de Andrade Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/26508-3 - Phylogeny of the hyperdiverse order Characiformes (Teleostei: Ostariophysi) using ultraconserved elements
Grantee:Claudio de Oliveira
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/09204-6 - Biodiversity and Biogeography in marine-estuarine fishes (Teleostei) from Brazil
Grantee:Claudio de Oliveira
Support type: Regular Research Grants