| Full text | |
| Author(s): |
Vanessa Silvestre Innocenti Giorgi
[1]
;
Rui Alberto Ferriani
;
Paula Andrea Navarro
Total Authors: 3
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Gynecology and Obstetrics - Brasil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Ginecologia e Obstetrícia; v. 43, n. 2, p. 119-125, 2021-03-29. |
| Abstract | |
Abstract Objective To investigate whether follicular fluid (FF) from infertile women with mild endometriosis (ME) alters in vitro bovine embryo development, and whether the antioxidants N-acetyl-cysteine (NAC) and/or L-carnitine (LC) could prevent such damages. Methods Follicular fluid was obtained from infertile women (11 with ME and 11 control). Bovine oocytes were matured in vitro divided in: No-FF, with 1% of FF from control women (CFF) or ME women (MEFF); with 1.5mM NAC (CFF + NAC, MEFF + NAC), with 0.6mg/mL LC (CFF + LC, MEFF + LC), or both antioxidants (CFF + NAC + LC, MEFF + NAC + LC). After in vitro fertilization, in vitro embryo culture was performed for 9 days. Results A total of 883 presumptive zygotes were cultured in vitro. No differences were observed in cleavage rate (p = 0.5376) and blastocyst formation rate (p = 0.4249). However, the MEFF group (12.5%) had lower hatching rate than the No-FF (42.1%, p = 0.029) and CFF (42.9%, p = 0.036) groups. Addition of antioxidants in the group with CFF did not alter hatching rate (p ≥ 0.56), and in groups with MEFF, just NAC increased the hatching rate [(MEFF: 12.5% versus MEFF + NAC: 44.4% (p = 0.02); vs MEFF + LC: 18.8% (p = 0.79); versus MEFF + NAC + LC: 30.8% (p = 0.22)]. Conclusion Therefore, FF from infertile women with ME added to medium of in vitro maturation of bovine oocytes impairs hatching rate, and NAC prevented these damages, suggesting involvement of oxidative stress in worst of oocyte and embryo quality of women with ME. (AU) | |
| FAPESP's process: | 12/15070-1 - Evaluation of the effect of antioxidants on in vitro maturation medium supplemented with follicular fluid of infertile women with endometriosis on oocyte and embryo quality using experimental bovine model |
| Grantee: | Vanessa Silvestre Innocenti Giorgi |
| Support Opportunities: | Scholarships in Brazil - Master |