Effects of different branched-chain amino acids supplementation protocols on the inflammatory response of LPS-stimulated RAW 264.7 macrophages

Author(s)

Bonvini, Andrea ^[1] ; Rogero, Marcelo Macedo ^[2] ; Coqueiro, Audrey Yule ^[1] ; Raizel, Raquel ^[1] ; Bella, Leonardo Mendes ^[3] ; Fock, Ricardo Ambrosio ^[3] ; Borelli, Primavera ^[3] ; Tirapegui, Julio ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Ave Prof Lineu Prestes 580, BR-05508000 Butanta, SP - Brazil

^[2] Univ Sao Paulo, Fac Publ Hlth, Dept Nutr, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil

• Document type: Journal article
• Source: Amino Acids; v. 53, n. 4, SI MAR 2021.
• Web of Science Citations: 0

Abstract

Although branched-chain amino acids (BCAA) are commonly used as a strategy to recover nutritional status of critically ill patients, recent findings on their role as immunonutrients have been associated with unfavorable outcomes, especially in obese patients. The present study aimed to explore the effects of different BCAA supplementation protocols in the inflammatory response of LPS-stimulated RAW 264.7 macrophages. Cell cultures were divided into five groups, with and without BCAA supplementation, (2 mmol/L of each amino acid). Then, cell cultures followed three different treatment protocols, consisting of a pretreatment (PT), an acute treatment (AT), and a chronic treatment (CT) with BCAA and LPS stimulation (1 mu g/mL). Cell viability was analyzed by MTT assay, NO production was assessed by the Griess reaction and IL-6, IL-10, TNF-alpha and PGE2 synthesis, was evaluated by ELISA. BCAA significantly increased cell viability in AT and CT protocols, and NO and IL-10 synthesis in all treatment protocols. IL-6 synthesis was only increased in PT and CT protocols. TNF-alpha and PGE2 synthesis were not altered in any of the protocols and groups. BCAA supplementation was able to increase both pro and anti-inflammatory mediators synthesis by RAW 264.7 macrophages, which was influenced by the protocol applied. Moreover, these parameters were significantly increased by isoleucine supplementation, highlighting a potential research field for future studies. (AU)

• FAPESP's process: 16/11360-6 - Effects of branched chain amino acids in the inflammatory response induced by lipopolysaccharide (LPS) in a macrophage cell line raw 264.7
• Grantee: Andrea Bonvini
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/04910-0 - Effect of chronic supplementation with L-glutamine and L-alanine, in free form or as dipeptide, on parameters associated with fatigue in rats subjected to resistance training.
• Grantee: Julio Orlando Tirapegui Toledo
• Support Opportunities: Regular Research Grants