| Full text | |
| Author(s): |
Floriano, Barbara Freitas
[1]
;
Carvalho, Tamara
[1]
;
Lopes, Tairine Zara
[1]
;
Unten Takahashi, Luandra Aparecida
[2]
;
Rahal, Paula
[1]
;
Tedesco, Antonio Claudio
[2]
;
Calmon, Marilia Freitas
[1]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo State Univ, IBILCE Inst Biosci Humanities & Exact Sci, Dept Biol, Rua Cristovao Colombo 2265, BR-15054010 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Languages Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, USP, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Photodiagnosis and Photodynamic Therapy; v. 33, MAR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Cervical carcinoma is the most common gynecological cancer among young and adult women. There has been increasing interest in natural sources for cervical carcinoma treatment, especially for active compounds from plant extracts as antineoplastic agents. Berberine is an example of one these promising natural products. It is a natural isoquinoline alkaloid and comes from plants, such as Berbis, Coptis, and Hydrastis. It is widely used in Chinese medicine and has demonstrated activity against various cancer cell lines. This work aims to analyze the efficiency of berberine-containing nanoemulsions as photosensitizing agents in photodynamic therapy and their interaction with cervical carcinoma cells and immortalized human keratinocyte cell line. Among all groups tested, berberine nanoemulsions combined with photodynamic therapy induced the most statistically significant phototoxicity in the evaluated cell lines. Fluorescence microscopy demonstrated that the compound was present for up to 48 h when berberine nanoemulsions were used. The reactive oxygen species assay showed an increase in reactive oxygen species in the two studied cell lines after treatment of berberine-containing nanoemulsion combined with photodynamic therapy. The autophagy trial showed significant increases in cell death when berberine-containing nanoemulsion treatment was combined with photodynamic therapy when compared to trichostatin A treatment as a positive control. However, caspase-3 activity did not significantly increase in cervical carcinoma cells and immortalized human keratinocyte cell line. The results suggest that nanoemulsions with berberine have potential for use as photosensitizing agents in photodynamic therapy to treat cervical carcinoma. (AU) | |
| FAPESP's process: | 15/16660-5 - Analysis of the nanoemulsions associated with photodynamic therapy in the treatment of breast cancer |
| Grantee: | Marilia de Freitas Calmon |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders |
| Grantee: | Antonio Claudio Tedesco |
| Support Opportunities: | Research Projects - Thematic Grants |