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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular and serological detection of arthropod-borne pathogens in carnivorous birds from Brazil

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Author(s):
Vieira Sacchi, Ana Beatriz [1] ; Andre, Marcos Rogerio [1] ; Calchi, Ana Claudia [1] ; de Santi, Mariele [1] ; Guimaraes, Andresa [2] ; Pires, Jeferson Rocha [3] ; Baldani, Cristiane Divan [2] ; Werther, Karin [1] ; Machado, Rosangela Zacarias [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, Lab Imunoparasitol, Dept Patol Reprod & Saude Unica, Vet, Fac Ciencias Agr & Vet, UNESP, Jaboticabal, SP - Brazil
[2] Univ Fed Rural Rio de Janeiro UFRRJ, Lab Patol Clin Diagnost Anim, Dept Med & Cirurgia Vet, Inst Vet, Seropedica, RJ - Brazil
[3] Univ Estacio Sa UNESA, Ctr Recuperacao Anim Silvestres GRAS, Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS; v. 23, JAN 2021.
Web of Science Citations: 0
Abstract

Rickettsiales, Haemosporida and Rhizobiales agents can cause diseases that affect various animal species, including humans. Due to predation behaviour, carnivorous birds may play an important role in spreading these etiological agentes across geographically distant areas, specially if they are migratory. The aim of this study was to investigate the occurrence and to access the phylogenetic relations among Anaplasmataceae (Ehrlichia, Anaplasma, Neorickettsia), Bartonellaceae (Bartonella spp.), and Haemosporida (Plasmodium, Haemoproteus and Leucocytozoon) agents in blood samples from 121 carnivorous birds sampled in the states of Sao Paulo and Rio de Janeiro, Brazil. Inclusions resembling hemoparasites were not observed in Giemsa-stained preparations. While three animals were seropositive for E. chaffeensis (3.41% {[}3/88]; 95% CI:1.17-9.55%), five showed antibodies to A. phagocytophilum (5.68% {[}5/88]; 95% CI: 2.45-12.62%). Despite the detection of rrs gene fragments closely related to E. chaffeensis (4.13% {[}5/121]; 95% CI: 1.78-9.31%), no positivity was observed in the qPCR based on the genes vlpt for the organism. Similarly, 12 (9.91% {[}12/121]; 95% CI: 5.76-16.74%) samples were positive in the qPCR for Anaplasma spp. based on groEL gene, but negative in the qPCR for A. phagocytophilum based on msp2 gene. Three samples were positive in the nPCR for E. canis based on rrs gene. Three samples were positive for Haemoproteus spp. and one for Plasmodium spp. in the nPCR based on cytB gene. Four birds (3.3% {[}4/121]; 95% CI: 1.29-8.19%) presented co-positivity by Ehrlichia sp. and Anaplasma sp. in molecular assays. One (0.82% {[}1/ 121]; 95% CI:0.15-4.53%) bird showed to be seropositive for E. chaffeensis and and positive in PCR for Haemoproteus sp. All birds were negative in the qPCR assay for Bartonella spp. (nuoG). The present work showed the occurrence of Anaplasmataceae agents and hemosporidians in carnivorous birds from southeastern Brazil. The role of these animals in the dispersion of Anaplasmataceae agents should be further investigated. (AU)

FAPESP's process: 12/11870-3 - Serological detection and molecular characterization of agents transmitted by arthropod vectors in carnivorous birds in Brazil
Grantee:Rosangela Zacarias Machado
Support type: Regular Research Grants