| Full text | |
| Author(s): |
de Souza Melo, Carina Guimaraes
[1]
;
Zanoni, Jacqueline Nelisis
[2]
;
Garcia de Souza, Sara Raquel
[2]
;
Zignani, Isabela
[2]
;
Leite, Aline de Lima
[1]
;
Heubel, Alessandro Domingues
[1]
;
Colombo Martins Perles, Juliana Vanessa
[2]
;
Rabelo Buzalaf, Marilia Afonso
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Estadual Maringa, Dept Morphophysiol Sci, Maringa, Parana - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | NEUROTOXICITY RESEARCH; v. 39, n. 3 MAR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The enteric nervous system is responsible for controlling the gastrointestinal tract (GIT) functions. Enteric neuropathies are highly correlated to the development of several intestinal disturbances. Fluoride (F) is extensively applied for dental health improvement and its ingestion can promote systemic toxicity with mild to severe GIT symptomatology and neurotoxicity. Although F harmful effects have been published, there is no information regarding noxiousness of a high acute F exposure (25 mg F/kg) on enteric neurons and levels of expression of intestinal proteins in the duodenum. Quantitative proteomics of the duodenum wall associated to morphometric and quantitative analysis of enteric neurons displayed F effects of a high acute exposure. F-induced myenteric neuroplasticity was characterized by a decrease in the density of nitrergic neurons and morphometric alterations in the general populations of neurons, nitrergic neurons, and substance P varicosities. Proteomics demonstrated F-induced alterations in levels of expression of 356 proteins correlated to striated muscle cell differentiation; generation of precursor metabolites and energy; NADH and glutathione metabolic process and purine ribonucleoside triphosphate biosynthesis. The neurochemical role of several intestinal proteins was discussed specially related to the modulation of enteric neuroplasticity. The results provide a new perspective on cell signaling pathways of gastrointestinal symptomatology promoted by acute F toxicity. (AU) | |
| FAPESP's process: | 12/16840-5 - Fluoride toxicology: proteomic studies in gut and liver |
| Grantee: | Marília Afonso Rabelo Buzalaf |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/10233-7 - Evaluation of enteric innervation and proteomic analysis of small intestin of rats exposed to chronic or acute fluoride dose |
| Grantee: | Carina Guimarães de Souza Melo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |