Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Author(s)

de Paula Nogueira Cruz, Felipe ^{[1, 2, 3]} ; Ferreira de Paula, Ailton ^[1] ; Nogueira, Camila Tita ^[2] ; Marques de Andrade, Paulo Henrique ^{[1, 2]} ; Borges, Leonardo Maurici ^[4] ; Lacava, Paulo Teixeira ^{[1, 3]} ; Baratella da Cunha Camargo, Ilana Lopes ^[5] ; Anibal, Fernanda de Freitas ^{[2, 3]} ; Paiva de Sousa, Cristina ^{[1, 3]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Morphol & Pathol, Lab Microbiol & Biomol LaMiB, Sao Carlos - Brazil

^[2] Univ Fed Sao Carlos, Lab Inflammat & Infect Dis LIDI, Sao Carlos - Brazil

^[3] Univ Fed Sao Carlos, Biotechnol Grad Program, Sao Carlos - Brazil

^[4] Univ Fed Sao Carlos, Dept Bot, Lab Taxon & Plant Evolut TaxEP, Sao Carlos - Brazil

^[5] Univ Sao Paulo, Phys Inst Sao Carlos, Lab Mol Epidemiol & Microbiol, Sao Carlos - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MICROBIOLOGY; v. 2021, FEB 17 2021.
• Web of Science Citations: 0

Abstract

In this study, we report the isolation and identification of an endophytic strain of Burkholderia cepacia (COPS strain) associated with Polygala paniculata roots. Polygala plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. B. cepacia COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes aph(3 `)-IIa and bla(TEM-116), respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles gyrB, lepA, and phaC. The root endophyte B. cepacia COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 9341, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as Moniliophthora perniciosa, whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas Sclerotinia sclerotiorum and Ceratocystis paradoxa showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited S. epidermidis ATCC 35984, E. faecium ATCC 700221 (MIC values of 32 mu g/mL for both), E. faecalis ATCC 29212 (64 mu g/mL), and S. aureus ATCC 25923 (128 mu g/mL). We observed moderate antagonistic activity against A. baumannii ATCC 19606 and E. coli ATCC 25922 (both at 512 mu g/mL), as well as potent cytotoxic effects on Leishmania infantum and Leishmania major promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic B. cepacia strain associated with P. paniculata and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/13423-5 - Compounds produced by endophyte microorganisms isolated from Brazilian tropical savannah: bioactivity, association with a-Ag2WO4 and biotechnological applications
• Grantee: Cristina Paiva de Sousa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/04788-8 - Evaluation of activity of natural products and other compounds against multidrug resistant bacteria and their biofilms
• Grantee: Felipe de Paula Nogueira Cruz
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training