| Full text | |
| Author(s): |
Spano, Liliana Cruz
[1]
;
Guerrieri, Caroline Gastaldi
[1]
;
Bondi Volpini, Lays Paula
[1]
;
Schuenck, Ricardo Pinto
[1]
;
Goulart, Jaqueline Pegoretti
[2]
;
Boina, Elizabeth
[2]
;
Nascimento Recco, Celia Regina
[3]
;
Ribeiro-Rodrigues, Rodrigo
[1, 2]
;
dos Santos, Luis Fernando
[4]
;
Fumian, Tulio Machado
[5]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Espirito Santo, Dept Pathol, Hlth Sci Ctr, Vitoria, ES - Brazil
[2] Cent Publ Hlth Lab, State Hlth Secretariat, Vitoria, ES - Brazil
[3] Municipal Hlth Secretariat, Epidemiol Serv, Espirito Santo - Brazil
[4] Adolfo Lutz Inst, Ctr Bacteriol, Natl Reference Lab Escherichia Coli Enter Infect, Sao Paulo - Brazil
[5] Fiocruz MS, Lab Comparat & Environm Virol, Oswaldo Cruz Inst, Rio De Janeiro - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BMC Microbiology; v. 21, n. 1 MAR 29 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
BackgroundThis study describes the investigation of an outbreak of diarrhea, hemorrhagic colitis (HC), and hemolytic uremic syndrome (HUS) at a daycare center in southeastern Brazil, involving fourteen children, six staff members, six family members, and one nurse. All bacterial and viral pathogens detected were genetically characterized.ResultsTwo isolates of a strain of enterohemorrhagic Escherichia coli (EHEC) serotype O111:H8 were recovered, one implicated in a case of HUS and the other in a case of uncomplicated diarrhea. These isolates had a clonal relationship of 94% and carried the stx2a and eae virulence genes and the OI-122 pathogenicity island. The EHEC strain was determined to be a single-locus variant of sequence type (ST) 327. EHEC isolates were resistant to ofloxacin, doxycycline, tetracycline, ampicillin, and trimethoprim-sulfamethoxazole and intermediately resistant to levofloxacin and ciprofloxacin. Rotavirus was not detected in any samples, and norovirus was detected in 46.7% (14/30) of the stool samples, three of which were from asymptomatic staff members. The noroviruses were classified as the recombinant GII.4 Sydney {[}P16] by gene sequencing.ConclusionIn this outbreak, it was possible to identify an uncommon stx2a +EHEC O111:H8 strain, and the most recent pandemic norovirus strain GII.4 Sydney {[}P16]. Our findings reinforce the need for surveillance and diagnosis of multiple enteric pathogens by public health authorities, especially during outbreaks. (AU) | |
| FAPESP's process: | 17/00411-1 - SEARCH FOR NEW GENETIC MARKERS AND MULTI LOCUS SEQUENCE TYPING (MLST) OF ATYPICAL ENTEROPATHOGENIC AND SHIGA TOXIN-PRODUCING Escherichia COLI STRAINS ISOLATED IN BRAZIL |
| Grantee: | Luis Fernando dos Santos |
| Support Opportunities: | Regular Research Grants |