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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Integrative microRNAome analysis of skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu), and the hybrid tambacu, based on next-generation sequencing data

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Author(s):
Fantinatti, Bruno E. A. [1, 2, 3] ; Perez, Erika S. [1] ; Zanella, Bruna T. T. [1] ; Valente, Jessica S. [1] ; de Paula, Tassiana G. [1] ; Mareco, Edson A. [4] ; Carvalho, Robson F. [1] ; Piazza, Silvano [3] ; Denti, Michela A. [3] ; Dal-Pai-Silva, Maeli [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil
[2] Ninth July Univ UNINOVE, Bauru, SP - Brazil
[3] Univ Trento, Dept Cellular Computat & Integrat Biol CIBIO, Trento - Italy
[4] Univ Western Sao Paulo Unoeste, Presidente Prudente, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BMC Genomics; v. 22, n. 1 APR 6 2021.
Web of Science Citations: 0
Abstract

Background Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms. Here, we used small RNA sequencing to examine global miRNA expression in the genotypes pacu (PC), tambaqui (TQ), and hybrid tambacu (TC), (Juveniles, n = 5 per genotype), to better understand the relationship between tambacu and its parental species, and also to clarify the mechanisms involved in tambacu muscle growth and maintenance based on miRNAs expression. Results Regarding differentially expressed (DE) miRNAs between the three genotypes, we observed 8 upregulated and 7 downregulated miRNAs considering TC vs. PC; 14 miRNAs were upregulated and 10 were downregulated considering TC vs. TQ, and 15 miRNAs upregulated and 9 were downregulated considering PC vs. TQ. The majority of the miRNAs showed specific regulation for each genotype pair, and no miRNA were shared between the 3 genotype pairs, in both up- and down-regulated miRNAs. Considering only the miRNAs with validated target genes, we observed the miRNAs miR-144-3p, miR-138-5p, miR-206-3p, and miR-499-5p. GO enrichment analysis showed that the main target genes for these miRNAs were grouped in pathways related to oxygen homeostasis, blood vessel modulation, and oxidative metabolism. Conclusions Our global miRNA analysis provided interesting DE miRNAs in the skeletal muscle of pacu, tambaqui, and the hybrid tambacu. In addition, in the hybrid tambacu, we identified some miRNAs controlling important molecular muscle markers that could be relevant for the farming maximization. (AU)

FAPESP's process: 16/05009-4 - Comparative and integrative analysis of the transcriptome and the microRNAoma in skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) and its hybrid, tambacu: molecular, in silico, in vitro and in vivo approaches.
Grantee:Maeli Dal Pai
Support type: Regular Research Grants
FAPESP's process: 15/19985-2 - Integrative microRNAome analysis of muscle tissue of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) and the hybrid, tambacu.
Grantee:Bruno Evaristo de Almeida Fantinatti
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/15708-0 - Bioinformatics applied to miRNAs in fish species
Grantee:Bruno Evaristo de Almeida Fantinatti
Support type: Scholarships abroad - Research Internship - Post-doctor