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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Probiotic Effects ofLactobacillus paracasei28.4 to InhibitStreptococcus mutansin a Gellan-Based Formulation

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Author(s):
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de Alvarenga, Janaina Araujo [1] ; de Barros, Patricia Pimentel [1] ; de Camargo Ribeiro, Felipe [1] ; Rossoni, Rodnei Dennis [1] ; Garcia, Maira Terra [1] ; dos Santos Velloso, Marisol [1] ; Shukla, Shashank [2] ; Fuchs, Beth Burgwyn [3, 4] ; Shukla, Anita [2] ; Mylonakis, Eleftherios [3, 4] ; Junqueira, Juliana Campos [1]
Total Authors: 11
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil
[2] Brown Univ, Sch Engn, Providence, RI 02912 - USA
[3] Brown Univ, Providence, RI 02912 - USA
[4] Rhode Isl Hosp, Alpert Med Sch, Providence, RI - USA
Total Affiliations: 4
Document type: Journal article
Source: PROBIOTICS AND ANTIMICROBIAL PROTEINS; v. 13, n. 2, p. 506-517, APR 2021.
Web of Science Citations: 0
Abstract

Streptococcus mutansis considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects ofLactobacillus paracasei28.4-enriched gellan hydrogel materials to inhibitS.mutansin planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors.L.paracasei28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release ofL.paracaseicells over 24 h that was sufficient to inhibit the planktonic growth ofS.mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity againstS.mutansbiofilms, exhibiting a reduction of 0.57 to 1.54 log(10)in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation ofluxS,brpA,gbpB, andgtfBgenes. The gellan formulation containingL.paracasei28.4 exhibited probiotic effects for preventingS.mutansgrowth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention. (AU)

FAPESP's process: 16/25544-1 - Development of probiotic formulations for bucal candidiasis prevention: in vitro and in vivo study
Grantee:Felipe de Camargo Ribeiro
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/15529-8 - Effects of different probiotics formulations containing Lactobacillus paracasei 28.4 on the antibacterial activity, polysaccharides production and biofilms formation by Streptococcus mutans
Grantee:Janaina Araújo de Alvarenga
Support Opportunities: Scholarships in Brazil - Master