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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Propolis from southeastern Brazil produced by Apis mellifera affects innate immunity by modulating cell marker expression, cytokine production and intracellular pathways in human monocytes

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Conte, Fernanda Lopes [1] ; Santiago, Karina Basso [1] ; Conti, Bruno Jose [1] ; Cardoso, Eliza de Oliveira [1] ; Garcia Oliveira, Lucas Pires [1] ; Feltran, Georgia da Silva [1] ; Zambuzzi, Willian Fernando [1] ; Golim, Marjorie de Assis [2] ; Cruz, Maria Teresa [3] ; Sforcin, Jose Mauricio [1]
Total Authors: 10
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Botucatu Blood Ctr, Sch Med, Sao Paulo - Brazil
[3] Univ Coimbra, Fac Pharm, Ctr Neurosci & Cellular Biol, Coimbra - Portugal
Total Affiliations: 3
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 73, n. 2, p. 135-144, MAR 4 2021.
Web of Science Citations: 0

Objectives Propolis is a bee-made product used for centuries due to its diverse biological properties, including its immunomodulatory action. This work aimed at investigating whether propolis may affect monocyte functions challenged with retinoic acid (RA), B subunit of Escherichia coli heat-labile enterotoxin (EtxB), human melanoma-associated antigen-1 (MAGE-1) and lipopolysaccharide (LPS). Methods Monocytes from healthy donors were treated with the stimuli separately or in the presence of propolis. Cell viability was evaluated by MTT assay, cell marker expression was assessed by flow cytometry, cytokine production by ELISA, gene expression by RT-qPCR. Key findings Propolis alone maintained TLR-2, TLR-4, HLA-DR, CD40 and CD80 expression in the monocytes; however, its combination with either MAGE-1 or LPS decreased CD40 expression triggered by the stimuli. Propolis maintained RA action on cell marker expression. Propolis inhibited TNF-alpha (with either EtxB or MAGE-1) and IL-6 (with either RA or MAGE-1), and increased IL-10 (with MAGE-1) production. Propolis downmodulated LC3 expression induced by LPS. It also induced a lower NF-kB expression than control cells and its combination with RA induced a higher expression than the stimulus alone. Conclusions Propolis potentially affected innate immunity by downmodulating the monocytes pro-inflammatory activity. (AU)

Grantee:José Maurício Sforcin
Support type: Regular Research Grants